Detailed information of TA system TA195442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3136616..3137220
• Replicon: chromosome
• Accession: NZ_CP071128
• Organism: Mycobacterium tuberculosis strain 267/47W148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: JZL63_RS14785
• Protein ID: WP_003414492.1
• Coordinates: 3136616..3137008 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JZL63_RS14790
• Protein ID: WP_206281284.1
• Coordinates: 3137005..3137220 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZL63_RS14755	3131766..3132554	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
JZL63_RS14760	3132888..3133433	-	546	WP_003913758.1	DUF1802 family protein	-
JZL63_RS14765	3133705..3134589	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JZL63_RS14770	3134592..3135479	-	888	WP_206281283.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
JZL63_RS14775	3135784..3136329	-	546	WP_095794191.1	DUF1802 family protein	-
JZL63_RS14780	3136326..3136595	-	270	WP_206280367.1	DUF2277 family protein	-
JZL63_RS14785	3136616..3137008	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
JZL63_RS14790	3137005..3137220	-	216	WP_206281284.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
JZL63_RS14795	3137267..3138016	+	750	WP_206280368.1	enoyl-CoA hydratase	-
JZL63_RS14800	3138095..3139177	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
JZL63_RS14805	3139170..3140480	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
JZL63_RS14810	3140483..3141310	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
JZL63_RS14815	3141307..3142218	-	912	WP_206280369.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T195442 WP_003414492.1 NZ_CP071128:c3137008-3136616 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T195442 NZ_CP094723:c1639035-1638682 [Staphylococcus sp. IVB6214]
ATGAAGCGAGGTGACGTATACTTAGCTGATTTATCACCAGTACAGGGTTCTGAACAAGGGGGAGTTAGACCTGTCGTTAT
CATACAAAATGATACTGGTAACAAATATAGTCCGACTGTGATTGTAGCAGCAATCACAGGACGTATTAATAAAGCGAAGA
TTCCAACACATGTAGAAATTGAAAAAAAGAAATATAAACTCGACAAAGATTCTGTTATTTTGTTGGAGCAGATTCGGACA
GTTGATAAAAATCGATTGAAAGAGAAGCTGACTTTTTTATCCGATGAAAAGATGAAAGAAGTGAACAATGCACTTGGCAT
CAGTCTAGGGCTACAAGTGGTTCAATCAAGATAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT195442 WP_206281284.1 NZ_CP071128:c3137220-3137005 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT195442 NZ_CP094723:c1639206-1639036 [Staphylococcus sp. IVB6214]
ATGTCAGTATTCAATCAAGTAAAATTTAAAAATTTAGAGCAGTCATTAAAAGAAGGTTACGCCCAAATGGCTGACCTCAA
TCTCTCCCTAGCGACAGAAGCCTATTCAGTAGAGTGTGAGGCGTGCGATTGTAATGAATCTCACTTACTATCAAAGCATC
AGGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8