Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2869723..2870360 Replicon chromosome
Accession NZ_CP071128
Organism Mycobacterium tuberculosis strain 267/47W148

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag JZL63_RS13340 Protein ID WP_003413180.1
Coordinates 2869723..2870118 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag JZL63_RS13345 Protein ID WP_003413183.1
Coordinates 2870115..2870360 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JZL63_RS13300 2865126..2866337 + 1212 WP_206280297.1 alpha/beta hydrolase family protein -
JZL63_RS13305 2866464..2867123 + 660 WP_206280298.1 hypothetical protein -
JZL63_RS13310 2867120..2867782 + 663 WP_206280299.1 hypothetical protein -
JZL63_RS13315 2867779..2868057 + 279 WP_206280300.1 type II toxin-antitoxin system VapB family antitoxin -
JZL63_RS13320 2868150..2868563 + 414 WP_003413164.1 PIN domain nuclease -
JZL63_RS13325 2868602..2868859 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
JZL63_RS13330 2868856..2869233 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
JZL63_RS13335 2869249..2869623 - 375 WP_003413177.1 hypothetical protein -
JZL63_RS13340 2869723..2870118 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
JZL63_RS13345 2870115..2870360 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
JZL63_RS13350 2870771..2871190 - 420 WP_003413190.1 A24 family peptidase -
JZL63_RS13355 2871202..2872011 - 810 WP_003413193.1 shikimate dehydrogenase -
JZL63_RS13360 2872008..2873261 - 1254 WP_206280301.1 endolytic transglycosylase MltG -
JZL63_RS13365 2873254..2873766 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T195435 WP_003413180.1 NZ_CP071128:c2870118-2869723 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T195435 NZ_CP094721:c1675456-1675103 [Staphylococcus sp. IVB6227]
ATGAAGCGAGGTGACGTATACTTAGCTGATTTATCACCAGTACAGGGTTCTGAACAAGGGGGAGTTAGACCTGTCGTTAT
CATACAAAATGATACTGGTAACAAATATAGTCCGACTGTGATTGTAGCAGCAATCACAGGACGTATTAATAAAGCGAAGA
TTCCAACACATGTAGAAATTGAAAAAAAGAAATATAAACTCGACAAAGATTCTGTTATTTTGTTGGAGCAGATTCGGACA
GTTGATAAAAATCGATTGAAAGAGAAGCTGACTTTTTTATCCGATGAAAAGATGAAAGAAGTGAACAATGCACTTGGCAT
CAGTCTAGGGCTACAAGTGGTTCAATCAAGATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT195435 WP_003413183.1 NZ_CP071128:c2870360-2870115 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT195435 NZ_CP094721:c1675627-1675457 [Staphylococcus sp. IVB6227]
ATGTCAGTATTCAATCAAGTAAAATTTAAAAATTTAGAGCAGTCATTAAAAGAAGGTTACGCCCAAATGGCTGACCTCAA
TCTCTCCCTAGCGACAGAAGCCTATTCAGTAGAGTGTGAGGCGTGCGATTGTAATGAATCTCACTTACTATCAAAGCATC
AGGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References