Detailed information of TA system TA195433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2868150..2868859
• Replicon: chromosome
• Accession: NZ_CP071128
• Organism: Mycobacterium tuberculosis strain 267/47W148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ26
• Locus tag: JZL63_RS13320
• Protein ID: WP_003413164.1
• Coordinates: 2868150..2868563 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: JZL63_RS13325
• Protein ID: WP_003413167.1
• Coordinates: 2868602..2868859 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZL63_RS13290	2863203..2864408	-	1206	WP_206280295.1	chorismate synthase	-
JZL63_RS13295	2864516..2864830	+	315	WP_206280296.1	hypothetical protein	-
JZL63_RS13300	2865126..2866337	+	1212	WP_206280297.1	alpha/beta hydrolase family protein	-
JZL63_RS13305	2866464..2867123	+	660	WP_206280298.1	hypothetical protein	-
JZL63_RS13310	2867120..2867782	+	663	WP_206280299.1	hypothetical protein	-
JZL63_RS13315	2867779..2868057	+	279	WP_206280300.1	type II toxin-antitoxin system VapB family antitoxin	-
JZL63_RS13320	2868150..2868563	+	414	WP_003413164.1	PIN domain nuclease	Toxin
JZL63_RS13325	2868602..2868859	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
JZL63_RS13330	2868856..2869233	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
JZL63_RS13335	2869249..2869623	-	375	WP_003413177.1	hypothetical protein	-
JZL63_RS13340	2869723..2870118	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
JZL63_RS13345	2870115..2870360	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
JZL63_RS13350	2870771..2871190	-	420	WP_003413190.1	A24 family peptidase	-
JZL63_RS13355	2871202..2872011	-	810	WP_003413193.1	shikimate dehydrogenase	-
JZL63_RS13360	2872008..2873261	-	1254	WP_206280301.1	endolytic transglycosylase MltG	-
JZL63_RS13365	2873254..2873766	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15045.00 Da
• Isoelectric Point: 5.4673

>T195433 WP_003413164.1 NZ_CP071128:2868150-2868563 [Mycobacterium tuberculosis]
MVFCVDTSAWHHAARPEVARRWLAALSADQIGICDHVRLEILYSANSATDYDALADELDGLARIPVGAETFTRACQVQRE
LAHVAGLHHRSVKIADLVIAAAAELSGTIVWHYDENYDRVAAITGQPTEWIVPRGTL

Nucleotide

• Length: 414 bp

>T195433 NZ_CP094720:c1779328-1779107 [Staphylococcus sp. IVB6233]
GTGCACAGCAAAGTATGGTTATCAGGTCTCTCGAGTGAGGAGCAAAAGCAACAGCTTGAATCGCGCCTGAGAGATATGAT
TGGTGTTGAAACGGCAGAGGTCGACATCGACAAGCAAATTGTGATTTTGACGTATGAAACCCCAGCCAATCTAAACACAT
TAGAAAAAGAAATTTATGATGCAGGTTACCCTGTTATTAATTCATATAAAGGAGAAGATTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT195433 WP_003413167.1 NZ_CP071128:2868602-2868859 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT195433 NZ_CP094720:c1779629-1779342 [Staphylococcus sp. IVB6233]
ATGATAGAACATGCGAATCATTCTGAAGTGACAAAACGCAATTTGCAATCCCGTTTAAATCGCATTGAAGGTCAAGTGCG
TGCAATTAATCGTATGATTGAAGAAGATGTTTATTGTGATGATGTCTTAACACAAATTCGTGCTACGAGATCGGCTTTAA
ATAGTGTAGCTTCGAAATTGTTAGAACATCATATGAAAGGGTGTATTTTGCATAAAGTTGAAGGTGGGAATGAGCAAGAA
GCAATGGATGAATTGCTTGTGACATTCCAAAAGTTGATGAAAGATTAG

Structures

Toxin

• AlphaFold DB: G0TQ26

Antitoxin

• AlphaFold DB: A0A829C9W5