Detailed information of TA system TA195432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2854263..2854903
• Replicon: chromosome
• Accession: NZ_CP071128
• Organism: Mycobacterium tuberculosis strain 267/47W148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: JZL63_RS13235
• Protein ID: WP_003412970.1
• Coordinates: 2854263..2854682 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: JZL63_RS13240
• Protein ID: WP_003412975.1
• Coordinates: 2854679..2854903 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZL63_RS13205	2849853..2850575	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
JZL63_RS13210	2851092..2851314	+	223	Protein_2607	type II toxin-antitoxin system VapB family antitoxin	-
JZL63_RS13215	2851311..2851712	+	402	WP_003412963.1	PIN domain-containing protein	-
JZL63_RS13220	2851747..2852667	-	921	WP_003412965.1	restriction endonuclease	-
JZL63_RS13225	2853008..2853253	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JZL63_RS13230	2853312..2854262	+	951	WP_003911916.1	Lsr2 family protein	-
JZL63_RS13235	2854263..2854682	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
JZL63_RS13240	2854679..2854903	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
JZL63_RS13245	2854934..2857777	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
JZL63_RS13250	2857849..2858250	-	402	WP_003412981.1	hypothetical protein	-
JZL63_RS13255	2858250..2858720	-	471	WP_003899364.1	transcription antitermination factor NusB	-
JZL63_RS13260	2858723..2859286	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T195432 WP_003412970.1 NZ_CP071128:c2854682-2854263 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T195432 NZ_CP094720:c1765176-1764823 [Staphylococcus sp. IVB6233]
ATGAAGCGCGGGGATGTATACTTAGCTGATTTATCACCAGTACGGGGTTCTGAACAAGGGGGAGTGAGACCCGTCGTTAT
CATTCAAAATGATACTGGTAACAAATATAGTCCGACTGTTATCGTGGCAGCGATAACAGGTCGTATTAACAAAGCAAAGA
TTCCAACACATGTTGAAATTGAAAAAAAGAAGTATAAGCTCGACAAAGATTCAGTTATTTTATTAGAACAAATTCGAACA
GTAGATAAAAATCGTTTGAAAGAAAAGTTGACTTTTTTATCAGAAGAAAAGATGAAAGAAGTGAACAATGCTTTGGGGAT
TAGTCTTGGCTTACAAGTTGTTCAATCACGATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT195432 WP_003412975.1 NZ_CP071128:c2854903-2854679 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT195432 NZ_CP094720:c1765347-1765177 [Staphylococcus sp. IVB6233]
ATGTCAGTATTCAATCAAGTGAAGTTTAAAAATTTAGAGCAGTCATTAAAAGAAGGGTACGCCCAAATGGCTGACCTCAA
TCTCTCCCTAGCGACAGAAGCATATTCAGTAGAGTGTGAAGCATGCGATTGCAATGAGTCACACTTATTATCCAAGCATC
AGGATGATTAA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09