Detailed information of TA system TA195431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2808084..2808736
• Replicon: chromosome
• Accession: NZ_CP071128
• Organism: Mycobacterium tuberculosis strain 267/47W148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JZL63_RS13040
• Protein ID: WP_206280283.1
• Coordinates: 2808311..2808736 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ24
• Locus tag: JZL63_RS13035
• Protein ID: WP_003412749.1
• Coordinates: 2808084..2808305 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZL63_RS13020	2806324..2806530	-	207	WP_003899347.1	hypothetical protein	-
JZL63_RS13025	2806666..2807289	+	624	WP_003900858.1	TIGR00725 family protein	-
JZL63_RS13030	2807279..2808031	+	753	WP_206280282.1	hypothetical protein	-
JZL63_RS13035	2808084..2808305	+	222	WP_003412749.1	antitoxin	Antitoxin
JZL63_RS13040	2808311..2808736	+	426	WP_206280283.1	PIN domain-containing protein	Toxin
JZL63_RS13045	2808759..2809940	-	1182	WP_206280284.1	2-oxo acid dehydrogenase subunit E2	-
JZL63_RS13050	2809937..2810983	-	1047	WP_003412757.1	3-methyl-2-oxobutanoate dehydrogenase subunit beta	-
JZL63_RS13055	2810994..2812097	-	1104	WP_003412761.1	pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha	-
JZL63_RS13060	2812356..2813177	-	822	WP_003412766.1	citrate (pro-3S)-lyase subunit beta	-
JZL63_RS13065	2813174..2813731	-	558	WP_003412768.1	MaoC family dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-139)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15422.88 Da
• Isoelectric Point: 8.1220

>T195431 WP_206280283.1 NZ_CP071128:2808311-2808736 [Mycobacterium tuberculosis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRESTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Nucleotide

• Length: 426 bp

>T195431 NZ_CP094720:c1544420-1544262 [Staphylococcus sp. IVB6233]
ATGAATAAACAAGAAAATGAACATGGCATGGTTGACAGCTTTGATGAAGTTGTTGAGTTAGGTAAAGAGATGGAACAAAT
CTCTGAAGAAAATGATGAAGAAAAGTATGAACAGTCACACGAGGAAGCGGTTCGTAGTGATCGTGAGACTGACGAATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 7864.93 Da
• Isoelectric Point: 4.5258

>AT195431 WP_003412749.1 NZ_CP071128:2808084-2808305 [Mycobacterium tuberculosis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Nucleotide

• Length: 222 bp

>AT195431 NZ_CP094720:c1545038-1544439 [Staphylococcus sp. IVB6233]
ATGGCAATGAACTTTAAAATTTTCAAAGATCAAAACACAGCTGCAACATTTGTAGCAGATATTTTAAGAAAACAATTTAG
CAATAATCCAACAACAATTGCAGGGATTCATTTAAAAAATGATGATGCACCGGTTAATGGCGCTTTACAAATTAATGTTG
AAAATAATCCGGTAGATTTTAGTCAAATTCACATTTTAGACTATGATAAAAATGATGCATATTTTAAAGCATTAGGTGTA
CCTGAGAAACAAATTCACGAAATCCCAGAAGATGAAAAAGTAGAAGCATTCATCAAGCATCATGCAAAAACAAAAGAAAA
TAAAGGTAAGCTAACGTTACAAGTCGTGACAATTGATACAGATGGAAGTATTGGTATTCCAATGAATGATGCTGTGTTAA
CGGCACGTGAAATCGTTCTTGTATTAACAGGTGCTGAAAAAGCTGAAGTGGTACGTAAGTTATACGAAGAAAACGGCAGT
ACAACATTTGTCCCATCAAGTTTAAAATCTCATCGTATGGTCAATGTTGTTTTAGATGAAGCAGCAGCACAAGGCTTACC
AGAAGATGTTAGAGCTTATTTCTCAGCACGTTTTGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BW03