Detailed information of TA system TA195428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK(toxin)
• Location: 2320828..2321464
• Replicon: chromosome
• Accession: NZ_CP071128
• Organism: Mycobacterium tuberculosis strain 267/47W148

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JZL63_RS10755
• Protein ID: WP_206280185.1
• Coordinates: 2321054..2321464 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P9WJ84
• Locus tag: JZL63_RS10750
• Protein ID: WP_003410651.1
• Coordinates: 2320828..2321061 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZL63_RS10740	2316678..2317082	-	405	WP_003410645.1	PPOX class F420-dependent oxidoreductase	-
JZL63_RS10745	2317166..2320750	-	3585	WP_206280184.1	cobaltochelatase subunit CobN	-
JZL63_RS10750	2320828..2321061	+	234	WP_003410651.1	type II toxin-antitoxin system antitoxin MazE7	Antitoxin
JZL63_RS10755	2321054..2321464	+	411	WP_206280185.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JZL63_RS10760	2321448..2322539	+	1092	WP_206280186.1	precorrin-3B synthase	-
JZL63_RS10765	2322549..2323175	+	627	WP_206280187.1	precorrin-8X methylmutase	-
JZL63_RS10770	2323172..2324698	+	1527	WP_003410659.1	precorrin-2 C(20)-methyltransferase	-
JZL63_RS10775	2324644..2325867	-	1224	WP_003901321.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14235.42 Da
• Isoelectric Point: 10.6228

>T195428 WP_206280185.1 NZ_CP071128:2321054-2321464 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Nucleotide

• Length: 411 bp

>T195428 NZ_CP094719:c1514914-1514756 [Staphylococcus sp. IVB6238]
ATGAATAAACAAGAAAATGAACATGGCATGGTTGACAGCTTTGATGAAGTTGTTGAGTTAGGTAAAGAGATGGAACAAAT
CTCTGAAGAAAATGATGAAGAAAAGTATGAACAGTCACACGAGGAAGCGGTTCGTAGTGATCGTGAGACTGACGAATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8450.25 Da
• Isoelectric Point: 4.7954

>AT195428 WP_003410651.1 NZ_CP071128:2320828-2321061 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Nucleotide

• Length: 234 bp

>AT195428 NZ_CP094719:c1515532-1514933 [Staphylococcus sp. IVB6238]
ATGGCAATGAACTTTAAAATTTTCAAAGATCAAAACACAGCTGCAACATTTGTAGCAGATATTTTAAGAAAACAATTTAG
CAATAATCCAACAACAATTGCAGGGATTCATTTAAAAAATGATGATGCACCGGTTAATGGCGCTTTACAAATTAATGTTG
AAAATAATCCGGTAGATTTTAGTCAAATTCACATTTTAGACTATGATAAAAATGATGCATATTTTAAAGCATTAGGTGTA
CCTGAGAAACAAATTCACGAAATCCCAGAAGATGAAAAAGTAGAAGCATTCATCAAGCATCATGCAAAAACAAAAGAAAA
TAAAGGTAAGCTAACGTTACAAGTCGTGACAATTGATACAGATGGAAGTATTGGTATTCCAATGAATGATGCTGTGTTAA
CGGCACGTGAAATCGTTCTTGTATTAACAGGTGCTGAAAAAGCTGAAGTGGTACGTAAGTTATACGAAGAAAACGGCAGT
ACAACATTTGTCCCATCAAGTTTAAAATCTCATCGTATGGTCAATGTTGTTTTAGATGAAGCAGCAGCACAAGGCTTACC
AGAAGATGTTAGAGCTTATTTCTCAGCACGTTTTGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BV50