Detailed information of TA system TA195426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2258042..2258668
• Replicon: chromosome
• Accession: NZ_CP071128
• Organism: Mycobacterium tuberculosis strain 267/47W148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: JZL63_RS10480
• Protein ID: WP_003410075.1
• Coordinates: 2258270..2258668 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: JZL63_RS10475
• Protein ID: WP_003911750.1
• Coordinates: 2258042..2258269 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZL63_RS10465	2256081..2256425	-	345	WP_206280166.1	4Fe-4S binding protein	-
JZL63_RS10470	2256614..2257783	-	1170	WP_003899126.1	ATP-binding protein	-
JZL63_RS10475	2258042..2258269	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JZL63_RS10480	2258270..2258668	+	399	WP_003410075.1	PIN domain nuclease	Toxin
JZL63_RS10485	2258851..2259282	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
JZL63_RS10490	2259383..2259817	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
JZL63_RS10495	2260254..2260433	-	180	Protein_2074	hypothetical protein	-
JZL63_RS10500	2260494..2261141	+	648	WP_206281267.1	transposase	-
JZL63_RS10505	2261095..2261685	+	591	WP_003899131.1	IS110 family transposase	-
JZL63_RS10510	2261813..2263069	-	1257	WP_003410095.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T195426 WP_003410075.1 NZ_CP071128:2258270-2258668 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T195426 NZ_CP094718:c1676314-1676093 [Staphylococcus sp. IVB6240]
GTGCACAGCAAAGTATGGTTATCAGGTCTCTCGAGTGAGGAGCAAAAGCAACAGCTTGAATCGCGCCTGAGAGATATGAT
TGGTGTTGAAACGGCAGAGGTCGACATCGACAAGCAAATTGTGATTTTGACGTATGAAACCCCAGCCAATCTAAACACAT
TAGAAAAAGAAATTTATGATGCAGGTTACACTGTTATTAATTCATATAAAGGAGAAGATTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT195426 WP_003911750.1 NZ_CP071128:2258042-2258269 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT195426 NZ_CP094718:c1676615-1676328 [Staphylococcus sp. IVB6240]
ATGATAGAACATGCGAATCATTCTGAAGTGACAAAACGCAATTTGCAATCCCGTTTAAATCGCATTGAAGGTCAAGTGCG
TGCAATTAATCGTATGATTGAAGAAGATGTTTATTGTGATGATGTCTTAACACAAATTCGTGCTACGAGATCGGCTTTAA
ATAGTGTAGCTTCGAAATTGTTAGAACATCATATGAAAGGGTGTATTTTGCATAAAGTTGAAGGTGGGAATGAGCAAGAA
GCAATGGATGAATTGCTTGTGACATTCCAAAAGTTGATGAAAGATTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8