Detailed information of TA system TA195420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2194641..2195344
• Replicon: chromosome
• Accession: NZ_CP071128
• Organism: Mycobacterium tuberculosis strain 267/47W148

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: JZL63_RS10100
• Protein ID: WP_003409778.1
• Coordinates: 2194641..2194970 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: JZL63_RS10105
• Protein ID: WP_003409780.1
• Coordinates: 2194967..2195344 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZL63_RS10080	2191024..2192094	+	1071	WP_206281221.1	alpha/beta fold hydrolase	-
JZL63_RS10085	2192091..2192606	+	516	WP_003409718.1	flavin reductase family protein	-
JZL63_RS10090	2192603..2193664	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
JZL63_RS10095	2193661..2194431	+	771	WP_003409775.1	SDR family oxidoreductase	-
JZL63_RS10100	2194641..2194970	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JZL63_RS10105	2194967..2195344	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
JZL63_RS10110	2195341..2195931	-	591	WP_003409784.1	SEC-C domain-containing protein	-
JZL63_RS10115	2195986..2197350	+	1365	WP_206281222.1	DUF222 domain-containing protein	-
JZL63_RS10120	2197505..2197957	-	453	WP_003899095.1	lipoprotein	-
JZL63_RS10125	2198021..2198422	+	402	WP_003409869.1	hypothetical protein	-
JZL63_RS10130	2198415..2198597	-	183	WP_003409870.1	hypothetical protein	-
JZL63_RS10135	2198711..2199061	-	351	WP_206281223.1	hypothetical protein	-
JZL63_RS10140	2199072..2199974	-	903	WP_003899097.1	hypothetical protein	-
JZL63_RS10145	2199995..2200186	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T195420 WP_003409778.1 NZ_CP071128:c2194970-2194641 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T195420 NZ_CP094717:1527761-1528216 [Staphylococcus sp. IVB6246]
ATGGGATTATACGAAGAAACTTTAATACAACATGACTATATAGAAGTAAGAGAGACGAATGTACTTCCTGATGATTTAGA
TGGAGCCTGGCTAGGTGATCTAATATTAATTAAACGTCACCTACCAGAAAAGAAGAAAGTCGAAGTCCTTTTTGAAGAAT
TAGCACATCACAAACTTACATACGGTGACATCACCAATCAAAAACAATTCAACAACCGAAAATTCGAAAATTACGCAAGA
CGCTATTCATATGAAACGTCTGTGCCCTTGTCAGGTATAGTCGAGGCGTTTAAGCAAGGTGTACGTAATTTGTACGAGCT
TGCTAATTTTTTTGAAATTACAGAAGGTCATGTACTAGATTGTATTGCACATTATAAAAAGAAGTATGGTTTACAAGTTA
GATACGGTGAATATCTAATCAGATTTGAGCCATTAACAATATATAAAAGTGTTTAG

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT195420 WP_003409780.1 NZ_CP071128:c2195344-2194967 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT195420 NZ_CP094717:1527419-1527748 [Staphylococcus sp. IVB6246]
ATGAGAAACAATGATGAAATAATCACAATAATCAAAGCATCTATGAAAAAACAAAATTTGTCGCTTAGTGAATTGGCTCG
TCGTGTAGGAGTTGCTAAATCTGCTGTGTCACGTTATTTAAATTTAACAAGAGAATTTCCATTAAATCGAGCAGAAGATT
TTGCAAAAGCGCTTAGTATCAGTACAGAATATTTACTTGGTTTTGAAAAAAGTGAACAAAAACAAGAACAACCACAGCAC
CGCGCAGCTCATTTGGAAGGAGAGCTGACTGATGAAGAGTGGCAACGTGTGTTAGATTATGCAGATTATATTAGAAGTAG
ACGCAAATAA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8