Detailed information of TA system TA195408

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1024207..1025180
• Replicon: chromosome
• Accession: NZ_CP071128
• Organism: Mycobacterium tuberculosis strain 267/47W148

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: JZL63_RS04815
• Protein ID: WP_206280957.1
• Coordinates: 1024680..1025180 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: JZL63_RS04810
• Protein ID: WP_206280956.1
• Coordinates: 1024207..1024683 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZL63_RS04790	1020054..1021325	-	1272	WP_003404747.1	PPE family protein	-
JZL63_RS04795	1021340..1021639	-	300	WP_003404748.1	PE family protein	-
JZL63_RS04800	1022083..1023864	+	1782	WP_003404749.1	BCCT family transporter	-
JZL63_RS04805	1023861..1024118	+	258	WP_206280955.1	hypothetical protein	-
JZL63_RS04810	1024207..1024683	+	477	WP_206280956.1	DUF1778 domain-containing protein	Antitoxin
JZL63_RS04815	1024680..1025180	+	501	WP_206280957.1	GNAT family N-acetyltransferase	Toxin
JZL63_RS04825	1025493..1026812	-	1320	WP_003404757.1	IS256-like element IS1554 family transposase	-
JZL63_RS04830	1027100..1027681	+	582	WP_003404760.1	IS607-like element IS1535 family transposase	-
JZL63_RS04835	1027681..1029333	+	1653	WP_206280958.1	IS607 family element transposase accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1023861..1069772	45911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (74-150)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18014.53 Da
• Isoelectric Point: 7.8659

>T195408 WP_206280957.1 NZ_CP071128:1024680-1025180 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHLDFEISPTDPLHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T195408 NZ_CP094713:846739-847092 [Staphylococcus schleiferi]
ATGAAGCGCGGGGACGTTTACTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTAGTTAT
TATTCAGAATGACACTGGAAATAAATACAGTCCAACTGTTATTGTCGCAGCAATTACTGGTCGGATTAATAAAGCTAAAA
TACCAACGCATGTAGAAATAGAAAAAAATAAATATAAGCTCGACAAAGATTCTGTCATCTTATTAGAACAAATTCGAACG
GTCGATAAGAAGAGACTTAAAGAGAAATTAACATTTTTATCTGAAGAAAAGATGAAAGAAGTTAATTACGCACTTGGTAT
TAGTTTAGGACTGAACATGAATCATCATAAATAG

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17387.98 Da
• Isoelectric Point: 11.2467

>AT195408 WP_206280956.1 NZ_CP071128:1024207-1024683 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGSSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 477 bp

>AT195408 NZ_CP094713:846568-846738 [Staphylococcus schleiferi]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAATCTTTAAAAGAAGGCTATGTTCAAATGGCAGATTTGAA
TCTCTCCCTAGCAACTGAAGCATATTCAGTTGAATGTGAAGCCTGTGATTGTAATGAATCACACTTGTTATCAGATTATA
AGGATGACTAA