Detailed information of TA system TA195406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 840946..841655
• Replicon: chromosome
• Accession: NZ_CP071128
• Organism: Mycobacterium tuberculosis strain 267/47W148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JZL63_RS03925
• Protein ID: WP_206280921.1
• Coordinates: 841227..841655 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TEX4
• Locus tag: JZL63_RS03920
• Protein ID: WP_003403834.1
• Coordinates: 840946..841203 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZL63_RS03915	838450..840855	+	2406	WP_206280920.1	PE domain-containing protein	-
JZL63_RS03920	840946..841203	+	258	WP_003403834.1	ribbon-helix-helix protein, CopG family	Antitoxin
JZL63_RS03925	841227..841655	+	429	WP_206280921.1	PIN domain-containing protein	Toxin
JZL63_RS03930	841745..841919	-	175	Protein_777	transposase	-
JZL63_RS03935	842032..842277	+	246	WP_003403841.1	hypothetical protein	-
JZL63_RS03940	842346..843230	-	885	WP_003403844.1	3-hydroxyisobutyrate dehydrogenase	-
JZL63_RS03945	843241..844413	-	1173	WP_003403845.1	acyl-CoA dehydrogenase FadE9	-
JZL63_RS03950	844420..845952	-	1533	WP_206280922.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-140)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15835.26 Da
• Isoelectric Point: 7.5536

>T195406 WP_206280921.1 NZ_CP071128:841227-841655 [Mycobacterium tuberculosis]
MFLLDANVLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLATNRRIFEIPSPRAEAFAFVEAVTAQPHHL
PTNPGPRHLMLLRKLCDEADASGDLIPDAVLAAIAVGHHCAVVSLDRDFARFASVRHIRPTL

Nucleotide

• Length: 429 bp

>T195406 NZ_CP094712:996515-996667 [Staphylococcus schleiferi]
ATGAAAGATGATCAAAAACAATATGAAAATGAAATGGTCGAAGGTTTTGATGATGTCGTTGAATTAGGCAAAGAGATGGA
ACAAATTTCAGAAAAAAATGATCAAGATAAATTAAATCAAGATCATGATGCTGATATTCGATCAGATAAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9114.28 Da
• Isoelectric Point: 10.6530

>AT195406 WP_003403834.1 NZ_CP071128:840946-841203 [Mycobacterium tuberculosis]
MRTTVSISDEILAAAKRRARERGQSLGAVIEDALRREFAAAHVGGARPTVPVFDGGTGPRRGIDLTSNRALSEVLDEGLE
LNSRK

Nucleotide

• Length: 258 bp

>AT195406 NZ_CP094712:995897-996496 [Staphylococcus schleiferi]
ATGGCAATGAATTTTAAAATATTTAAAGATGCAGAAACAGTGGCAAAATATACAGCAGATATCTTAAGAAAACAGCTTAA
TAATAATCCTACCTCAATTGTAGGTGTCCATTTAAATGAAGACCAAGCGCCGGTTTTAAATTTTTTAAAACAAGATGTTG
ATCGTCATAGTGTAGATTTTAGTCAAATTCATATTTTGGACTATGATAAGCAAGCGTCATTTTTTAAATCATTAGGCGTA
CCAGAGAAACAAGTGCATGAAGTTCCAGATGATGACAATGTTGAATCATTTATTGCCCATCATGCTAAAACAAAAGATAA
CAAAGGAAAATTAACGTTACAAGTATTAACTATTGATGAAAACGGACAAATTGGGTTACCAATGAATGATGCCGTAATGC
CAGCGCGTGAAATTATTGTCGTTGTCACAGGTTCAAATAAAGCGGCACAAGTTCAAAAGTTATATGAAGAAAATGGGAAT
ACAAGCTTTATTCCGTCAACATTAAAAACACATCGTATGGTGACTGTTGTCCTTGATGAAGCGGCTGCACAAGGTTTACC
AGATGATGTTAGAGCCTATTTTACTTCATTATATGCATAA

Structures

Antitoxin

• AlphaFold DB: G0TEX4