Detailed information of TA system TA195397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 697153..697799
• Replicon: chromosome
• Accession: NZ_CP071128
• Organism: Mycobacterium tuberculosis strain 267/47W148

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: JZL63_RS03115
• Protein ID: WP_003403137.1
• Coordinates: 697153..697566 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: JZL63_RS03120
• Protein ID: WP_003403139.1
• Coordinates: 697563..697799 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZL63_RS03095	693236..694786	+	1551	WP_206281232.1	MCE family protein	-
JZL63_RS03100	694838..695230	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
JZL63_RS03105	695227..695484	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
JZL63_RS03110	695667..696902	-	1236	WP_206280894.1	DUF4143 domain-containing protein	-
JZL63_RS03115	697153..697566	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
JZL63_RS03120	697563..697799	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JZL63_RS03125	697903..698409	-	507	WP_003900973.1	two component system sensor kinase HK1	-
JZL63_RS03130	698523..699053	-	531	WP_003917320.1	two component system sensor kinase HK2	-
JZL63_RS03135	699037..699732	-	696	WP_206281233.1	response regulator	-
JZL63_RS03140	699855..700166	+	312	WP_003403164.1	hypothetical protein	-
JZL63_RS03145	700238..701188	+	951	WP_206280895.1	DUF1259 domain-containing protein	-
JZL63_RS03150	701429..702013	+	585	WP_206280896.1	IS607 family transposase	-
JZL63_RS03155	702015..702728	+	714	Protein_625	IS607 family element transposase accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T195397 WP_003403137.1 NZ_CP071128:c697566-697153 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T195397 NZ_CP094706:1004647-1004997 [Staphylococcus simulans]
ATGATGAGGAGAGGCGATGTTTATTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGACCTGTTGT
AATTATACAAAATGACACTGGCAATAAGTACAGTCCGACGGTAATTGTAGCCGCGATTACTGGGCGTATTAATAAGGCGA
AAATTCCAACACATGTTGAAATTGAAAAGAGTAAATACAAATTAGACAAAGATTCTGTTATATTACTTGAACAGATTAGA
ACAGTTGATAAAAAACGTTTGAAAGAGAAATTGACTTATCTTTCAGATGAGAAGATGAAGGAAATTGATAACGCGATTGG
AATTAGTTTAGGCTTGACACATAAAAATTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT195397 WP_003403139.1 NZ_CP071128:c697799-697563 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT195397 NZ_CP094706:1004483-1004650 [Staphylococcus simulans]
ATGTCAACTTTTAATCAGAATAGAAGCTATAGTTTGGAACAGTCATTAAAAGAAGGCTATTCACAAATGGCTGATTTGAA
TCTCTCCCTAGCAACGGAGGCATTTTCAGTGGAATGTGAAGCCTGTGATTGCAATGAATCTTATCTTACATTTGATAAGG
ATGAATGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89