Detailed information of TA system TA195383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3183130..3183817
• Replicon: chromosome
• Accession: NZ_CP071127
• Organism: Mycobacterium tuberculosis strain 120/26CAO

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: JY387_RS15025
• Protein ID: WP_003414624.1
• Coordinates: 3183374..3183817 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: JY387_RS15020
• Protein ID: WP_003414620.1
• Coordinates: 3183130..3183387 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY387_RS15000	3178450..3179304	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
JY387_RS15005	3179360..3180523	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
JY387_RS15010	3180540..3181754	-	1215	WP_206280378.1	site-2 protease family protein	-
JY387_RS15015	3181762..3183003	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
JY387_RS15020	3183130..3183387	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
JY387_RS15025	3183374..3183817	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
JY387_RS15030	3183897..3184559	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
JY387_RS15035	3184655..3184846	+	192	WP_003414632.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
JY387_RS15040	3185178..3186926	+	1749	WP_206337966.1	redoxin domain-containing protein	-
JY387_RS15045	3187022..3187603	+	582	WP_003414644.1	fasciclin domain-containing protein	-
JY387_RS15050	3187703..3187969	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T195383 WP_003414624.1 NZ_CP071127:3183374-3183817 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T195383 NZ_CP094696:1026309-1026659 [Staphylococcus simulans]
ATGATGAGGAGAGGCGATGTTTATTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGACCTGTTGT
AATTATACAAAATGACACTGGCAATAAGTACAGTCCGACGGTAATTGTAGCCGCGATTACTGGGCGTATTAATAAGGCGA
AAATTCCAACACATGTTGAAATTGAAAAGAGTAAATACAAATTAGACAAAGATTCTGTTATATTACTTGAACAGATTAGA
ACAGTTGATAAAAAACGTTTGAAAGAGAAATTGACTTATCTTTCAGATGAGAAGATGAAAGAAATTGATAACGCGATTGG
AATTAGTTTAGGCTTGACACATAAAAATTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT195383 WP_003414620.1 NZ_CP071127:3183130-3183387 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT195383 NZ_CP094696:1026145-1026312 [Staphylococcus simulans]
ATGTCAACTTTTAATCAGAATAGAAGCTATAGTTTGGAACAGTCATTAAAAGAAGGCTATTCACAAATGGCTGATTTGAA
TCTCTCCCTAGCAACGGAGGCATTTTCAGTGGAATGTGAAGCCTGTGATTGCAATGAATCTTATCTTACATTTGATAAGG
ATGAATGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• AlphaFold DB: G0TFH0