Detailed information of TA system TA195382

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3177529..3178077
• Replicon: chromosome
• Accession: NZ_CP071127
• Organism: Mycobacterium tuberculosis strain 120/26CAO

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TFG5
• Locus tag: JY387_RS14995
• Protein ID: WP_003414602.1
• Coordinates: 3177814..3178077 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: O33347
• Locus tag: JY387_RS14990
• Protein ID: WP_003414599.1
• Coordinates: 3177529..3177810 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY387_RS14965	3173152..3174009	-	858	WP_206280375.1	type I methionyl aminopeptidase	-
JY387_RS14970	3174051..3174635	-	585	WP_206280376.1	DUF1707 domain-containing protein	-
JY387_RS14975	3174739..3174987	+	249	WP_003913411.1	antitoxin VapB23	-
JY387_RS14980	3174984..3175364	+	381	WP_003414592.1	type II toxin-antitoxin system VapC family toxin	-
JY387_RS14985	3175446..3177257	-	1812	WP_206337965.1	penicillin-binding transpeptidase domain-containing protein	-
JY387_RS14990	3177529..3177810	+	282	WP_003414599.1	type II toxin-antitoxin system antitoxin RelF	Antitoxin
JY387_RS14995	3177814..3178077	+	264	WP_003414602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JY387_RS15000	3178450..3179304	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
JY387_RS15005	3179360..3180523	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
JY387_RS15010	3180540..3181754	-	1215	WP_206280378.1	site-2 protease family protein	-
JY387_RS15015	3181762..3183003	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10204.82 Da
• Isoelectric Point: 11.5078

>T195382 WP_003414602.1 NZ_CP071127:3177814-3178077 [Mycobacterium tuberculosis]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Nucleotide

• Length: 264 bp

>T195382 NZ_CP094696:1013155-1013361 [Staphylococcus simulans]
ATGCATAGTAGTGTGATTCAAATCAGTGGTATGGAAACTGAAGAACAACAGCATATTTTGCAAGAACATTTGCAGCAAAT
GATTGGTATGTCAGAAGTGCAAGTGGATCTGAAATCACAAGAAGTGAAAGCGAGTTACCAGACACCAATCAGTTTAAACA
ATTTGGAAAAAGAAATTTATGATCAAGGTTACCAAATATTAAATTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10183.40 Da
• Isoelectric Point: 4.3440

>AT195382 WP_003414599.1 NZ_CP071127:3177529-3177810 [Mycobacterium tuberculosis]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Nucleotide

• Length: 282 bp

>AT195382 NZ_CP094696:1012847-1013140 [Staphylococcus simulans]
ATGACGCAAGAAGAACGCGCACATCATTCAGAAAAAATAAAATCTAACCTTAAAGCACGACTGAATCGTATAGAAGGACA
AGTCAGAGCGATTAATCGCATGATAGATGAAGATGTTTATTGTGATGATGTTTTAACACAAATCAGAGCGACACGTTCAG
CTTTGAATAGTGTTGCTGTAAAATTGCTGGATCATCATATGAAAGGCTGTATTTTGCATAAAGCTGAAGATGGACAAGCA
GAAGCTGCAATGGAAGAATTATTAGTTACCGTCCAAAAATTAATTAAAGATTAA

Structures

Toxin

• PDB: 3G5O

Antitoxin

• AlphaFold DB: A0A7U4BWP8