Detailed information of TA system TA195377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3070162..3070841
• Replicon: chromosome
• Accession: NZ_CP071127
• Organism: Mycobacterium tuberculosis strain 120/26CAO

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: JY387_RS14430
• Protein ID: WP_003414059.1
• Coordinates: 3070162..3070578 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JY387_RS14435
• Protein ID: WP_003414061.1
• Coordinates: 3070575..3070841 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY387_RS14410	3066214..3067116	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JY387_RS14415	3067185..3067937	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JY387_RS14420	3068181..3068456	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JY387_RS14425	3068453..3070075	-	1623	WP_096872274.1	type I restriction-modification system subunit M	-
JY387_RS14430	3070162..3070578	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
JY387_RS14435	3070575..3070841	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JY387_RS14440	3070867..3071262	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
JY387_RS14445	3071259..3071528	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JY387_RS14450	3071538..3072632	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
JY387_RS14455	3072629..3073048	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JY387_RS14460	3073122..3073601	-	480	WP_003414073.1	dihydrofolate reductase	-
JY387_RS14465	3073672..3074472	-	801	WP_206337957.1	thymidylate synthase	-
JY387_RS14470	3074628..3075365	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T195377 WP_003414059.1 NZ_CP071127:c3070578-3070162 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T195377 NZ_CP094677:2923767-2924048 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT195377 WP_003414061.1 NZ_CP071127:c3070841-3070575 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT195377 NZ_CP094677:2923543-2923770 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64