Detailed information of TA system TA195373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2868598..2869229
• Replicon: chromosome
• Accession: NZ_CP071127
• Organism: Mycobacterium tuberculosis strain 120/26CAO

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: JY387_RS13340
• Protein ID: WP_003413174.1
• Coordinates: 2868852..2869229 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: JY387_RS13335
• Protein ID: WP_003413167.1
• Coordinates: 2868598..2868855 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY387_RS13305	2864512..2864826	+	315	WP_009937839.1	hypothetical protein	-
JY387_RS13310	2865122..2866333	+	1212	WP_206337933.1	alpha/beta hydrolase family protein	-
JY387_RS13315	2866460..2867119	+	660	WP_206337934.1	hypothetical protein	-
JY387_RS13320	2867116..2867778	+	663	WP_206337935.1	hypothetical protein	-
JY387_RS13325	2867775..2868053	+	279	WP_206280300.1	type II toxin-antitoxin system VapB family antitoxin	-
JY387_RS13330	2868146..2868559	+	414	WP_003413164.1	PIN domain nuclease	-
JY387_RS13335	2868598..2868855	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
JY387_RS13340	2868852..2869229	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
JY387_RS13345	2869245..2869619	-	375	WP_003413177.1	hypothetical protein	-
JY387_RS13350	2869719..2870114	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
JY387_RS13355	2870111..2870356	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
JY387_RS13360	2870767..2871186	-	420	WP_003413190.1	A24 family peptidase	-
JY387_RS13365	2871198..2872007	-	810	WP_003413193.1	shikimate dehydrogenase	-
JY387_RS13370	2872004..2873257	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
JY387_RS13375	2873250..2873762	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T195373 WP_003413174.1 NZ_CP071127:2868852-2869229 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T195373 NZ_CP094676:c1234446-1234183 [Streptomyces sp. CB09030]
GTGAGTGAATACCGAACCGTCTTCCGGCCAGAGGCACAGGCCGAGCTCCGGAAGATCCCTCGCGACATGGCCCTACGCAT
CCTGACCAAACTGACCGAACTGGAGAGCGACCCCCTCGGCTATAGCACCACCGCGCTCGTGTCCCAGCCAGAACGCCGTC
GACTGCGGGTCGGCGACTACCGGGTCGTCTACACGATCGACAAGGGGGAGCTCGTGGTGTGGGTCGTCCACGTCGGGCAT
CGCTCCACCGTTTACGAAGCCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT195373 WP_003413167.1 NZ_CP071127:2868598-2868855 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT195373 NZ_CP094676:c1234708-1234439 [Streptomyces sp. CB09030]
ATGACGCAGCCACTGCCCATAGAGTCCATCCGCGACGTACGTGCGCACCTGGCCGAGGTCGTGGAGCGCGCCGATCGGGA
TGATGTGCCCACCGTGATCACTCGGCGGGGCAAGCAGATTGCCGCCGTCGTCTCCATCGAGGTGCTGCGCAAGTACCAGG
AATGGGAAGAGCGCGAGATCAATCGGATCATCGACGAACGCATGGCCAACCCGGCACCCGGCATCCCGATCGAGGAGATC
ATGAGGGAGACGCTGGCACGCGGTGAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5