Detailed information of TA system TA195371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2854259..2854899
• Replicon: chromosome
• Accession: NZ_CP071127
• Organism: Mycobacterium tuberculosis strain 120/26CAO

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: JY387_RS13245
• Protein ID: WP_003412970.1
• Coordinates: 2854259..2854678 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: JY387_RS13250
• Protein ID: WP_003412975.1
• Coordinates: 2854675..2854899 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY387_RS13215	2849849..2850571	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
JY387_RS13220	2851088..2851310	+	223	Protein_2609	type II toxin-antitoxin system VapB family antitoxin	-
JY387_RS13225	2851307..2851708	+	402	WP_003412963.1	PIN domain-containing protein	-
JY387_RS13230	2851743..2852663	-	921	WP_003412965.1	restriction endonuclease	-
JY387_RS13235	2853004..2853249	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JY387_RS13240	2853308..2854258	+	951	WP_206337932.1	Lsr2 family protein	-
JY387_RS13245	2854259..2854678	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
JY387_RS13250	2854675..2854899	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
JY387_RS13255	2854930..2857773	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
JY387_RS13260	2857845..2858246	-	402	WP_003412981.1	hypothetical protein	-
JY387_RS13265	2858246..2858716	-	471	WP_003899364.1	transcription antitermination factor NusB	-
JY387_RS13270	2858719..2859282	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T195371 WP_003412970.1 NZ_CP071127:c2854678-2854259 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T195371 NZ_CP094667:49646-49936 [Gordonia amicalis]
GTGAGCGACAACCCCACGGACTCGGCCGATGAGTACCGCGTCGAGGTCGCATCCCCCGCACGCCGCGATCTGCAACGCCT
TCCATCCCGGATCGTCCACGCTGTCATCGAGTTCATCTCCGGGCCCCTCGCCGGCAACCCGCACCGCCTCAGCAAACCGC
TACGCGACGACCTCGAAGGCCTTCACAGCGCACGCCGCGGCGACTACCGCATCCTGCTGCGCATCGACGATCCCCACCAC
ACCATCGTCATCGTCAAGATCGACCACCGAGCCCACGCCTACCGCACCTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT195371 WP_003412975.1 NZ_CP071127:c2854899-2854675 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT195371 NZ_CP094667:49374-49649 [Gordonia amicalis]
ATGACGACAGTGCCTCTCGGCGAAGCCAAGGACAAGCTCTCCGCGCTCATCGACAGCGCCGAGAGCACGCACGACATCAT
CACGATCACCAAACACGGCAAACCTGCCGCGGTGCTGATGTCGGCTGACGACCTCGAATCGCTGCACGAAACGATCTACT
GGCTCTCACGCGCCGGCATCCGCGAAGCTGTCGCGGCTGCTGACGTCGAGTACGCCGCCGGCCAAACCGTTTCCCTCGAG
GACCTGCGCGTCGAACACGGCATGCCGCCCCAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09