Detailed information of TA system TA195370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2808080..2808732
• Replicon: chromosome
• Accession: NZ_CP071127
• Organism: Mycobacterium tuberculosis strain 120/26CAO

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JY387_RS13050
• Protein ID: WP_206280283.1
• Coordinates: 2808307..2808732 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ24
• Locus tag: JY387_RS13045
• Protein ID: WP_003412749.1
• Coordinates: 2808080..2808301 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY387_RS13030	2806320..2806526	-	207	WP_003899347.1	hypothetical protein	-
JY387_RS13035	2806662..2807285	+	624	WP_003900858.1	TIGR00725 family protein	-
JY387_RS13040	2807275..2808027	+	753	WP_206280282.1	hypothetical protein	-
JY387_RS13045	2808080..2808301	+	222	WP_003412749.1	antitoxin	Antitoxin
JY387_RS13050	2808307..2808732	+	426	WP_206280283.1	PIN domain-containing protein	Toxin
JY387_RS13055	2808755..2809936	-	1182	WP_206337928.1	2-oxo acid dehydrogenase subunit E2	-
JY387_RS13060	2809933..2810979	-	1047	WP_003412757.1	3-methyl-2-oxobutanoate dehydrogenase subunit beta	-
JY387_RS13065	2810990..2812093	-	1104	WP_003412761.1	pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha	-
JY387_RS13070	2812352..2813173	-	822	WP_003412766.1	citrate (pro-3S)-lyase subunit beta	-
JY387_RS13075	2813170..2813727	-	558	WP_003412768.1	MaoC family dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-139)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15422.88 Da
• Isoelectric Point: 8.1220

>T195370 WP_206280283.1 NZ_CP071127:2808307-2808732 [Mycobacterium tuberculosis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRESTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Nucleotide

• Length: 426 bp

>T195370 NZ_CP094665:c3901322-3901032 [Gordonia amicalis]
ATGAGCGACGCTCCGACAGACTCGCCCGCCCCGTACCGGGTCGAGGTCGCATCCCCAGCTCGCCGCGACCTTCAACGCCT
TCCATCCCGGATCGTCCACGCCGTCATCGAATTCATCTCCGGACCTCTCGCTGGTAACCCGCACCGGCTCAGCAAGCCGC
TGCGCAACGACCTCGCCGGCCTCCACAGCGCCCGACGCGGCGACTACCGCGTCCTGCTCCGCATCGACGACCCGAACCAC
ACCATCGTTGTCGTCAGAATCGACCACCGTGCCCACGCCTATCGCACCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 7864.93 Da
• Isoelectric Point: 4.5258

>AT195370 WP_003412749.1 NZ_CP071127:2808080-2808301 [Mycobacterium tuberculosis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Nucleotide

• Length: 222 bp

>AT195370 NZ_CP094665:c3901594-3901319 [Gordonia amicalis]
ATGACGACAGTGCCACTGGGTGAAGCCAAGGACAAGCTCTCCGCGCTGGTCGACAGCGCCGAAACCACGCACGACATCAT
CACGATCACCAAGCACGGCAAACCCGCCGCGGTACTGATGTCGGCCGACGATCTCGAATCGCTGCACGAGACGATCTACT
GGCTCTCACGTGCCGGTGTCCGCGACGACGTCGCTACCGCCGACGCCGAGTACGCCGCCGGCCACACCGTCTCCATCGAC
GACCTGCGCGCCGAACACGGCATGCCGCCGAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BW03