Detailed information of TA system TA195365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2258041..2258667
• Replicon: chromosome
• Accession: NZ_CP071127
• Organism: Mycobacterium tuberculosis strain 120/26CAO

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: JY387_RS10485
• Protein ID: WP_003410075.1
• Coordinates: 2258269..2258667 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: JY387_RS10480
• Protein ID: WP_003911750.1
• Coordinates: 2258041..2258268 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY387_RS10470	2256080..2256424	-	345	WP_206337882.1	4Fe-4S binding protein	-
JY387_RS10475	2256613..2257782	-	1170	WP_003899126.1	ATP-binding protein	-
JY387_RS10480	2258041..2258268	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JY387_RS10485	2258269..2258667	+	399	WP_003410075.1	PIN domain nuclease	Toxin
JY387_RS10490	2258850..2259281	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
JY387_RS10495	2259382..2259816	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
JY387_RS10500	2260253..2260432	-	180	Protein_2075	hypothetical protein	-
JY387_RS10505	2260493..2261140	+	648	WP_206281267.1	transposase	-
JY387_RS10510	2261094..2261684	+	591	WP_003899131.1	IS110 family transposase	-
JY387_RS10515	2261812..2263068	-	1257	WP_003410095.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T195365 WP_003410075.1 NZ_CP071127:2258269-2258667 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T195365 NZ_CP094663:c1608977-1608768 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT195365 WP_003911750.1 NZ_CP071127:2258041-2258268 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT195365 NZ_CP094663:c1609282-1608989 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8