Detailed information of TA system TA195363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-TIGR02293
• Location: 2232735..2233633
• Replicon: chromosome
• Accession: NZ_CP071127
• Organism: Mycobacterium tuberculosis strain 120/26CAO

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: P64908
• Locus tag: JY387_RS10360
• Protein ID: WP_003410001.1
• Coordinates: 2232735..2233295 (-)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: P64910
• Locus tag: JY387_RS10365
• Protein ID: WP_003410003.1
• Coordinates: 2233292..2233633 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY387_RS10330	2227904..2228557	-	654	WP_003409976.1	cutinase Cfp21	-
JY387_RS10335	2228672..2228902	+	231	WP_003409980.1	DUF167 domain-containing protein	-
JY387_RS10340	2228987..2229898	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
JY387_RS10345	2230007..2230606	+	600	WP_206280157.1	L-lysine exporter	-
JY387_RS10350	2231022..2231450	+	429	WP_003409992.1	cellulose-binding protein	-
JY387_RS10355	2231676..2232215	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
JY387_RS10360	2232735..2233295	-	561	WP_003410001.1	RES family NAD+ phosphorylase	Toxin
JY387_RS10365	2233292..2233633	-	342	WP_003410003.1	DUF2384 domain-containing protein	Antitoxin
JY387_RS10370	2233719..2233976	+	258	WP_003410006.1	hypothetical protein	-
JY387_RS10375	2233877..2234212	-	336	WP_003410009.1	dehydrogenase	-
JY387_RS10380	2234301..2234645	-	345	WP_003410010.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	-
JY387_RS10385	2234639..2234887	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	-
JY387_RS10390	2234987..2237302	-	2316	WP_206337880.1	cadmium-translocating P-type ATPase	-
JY387_RS10395	2237299..2237571	-	273	WP_206280159.1	DUF1490 family protein	-
JY387_RS10400	2237624..2237980	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20248.70 Da
• Isoelectric Point: 4.5091

>T195363 WP_003410001.1 NZ_CP071127:c2233295-2232735 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Nucleotide

• Length: 561 bp

>T195363 NZ_CP094663:c1493735-1493559 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12488.33 Da
• Isoelectric Point: 4.8359

>AT195363 WP_003410003.1 NZ_CP071127:c2233633-2233292 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Nucleotide

• Length: 342 bp

>AT195363 NZ_CP094663:1493436-1493491 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Structures

Toxin

• PDB: 6FKG

Antitoxin

• AlphaFold DB: A0A7U4FAE4