Detailed information of TA system TA195349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1230656..1231287
• Replicon: chromosome
• Accession: NZ_CP071127
• Organism: Mycobacterium tuberculosis strain 120/26CAO

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JY387_RS05795
• Protein ID: WP_206281013.1
• Coordinates: 1230656..1230967 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TGZ7
• Locus tag: JY387_RS05800
• Protein ID: WP_003405836.1
• Coordinates: 1230967..1231287 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY387_RS05775	1226137..1227561	-	1425	WP_003405805.1	class II fumarate hydratase	-
JY387_RS05780	1227592..1228680	-	1089	WP_003898726.1	class II fructose-bisphosphatase	-
JY387_RS05785	1228736..1229380	+	645	WP_003915494.1	DUF4245 domain-containing protein	-
JY387_RS05790	1229387..1230544	-	1158	WP_003898727.1	AI-2E family transporter	-
JY387_RS05795	1230656..1230967	-	312	WP_206281013.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JY387_RS05800	1230967..1231287	-	321	WP_003405836.1	type II toxin-antitoxin system antitoxin MazE3	Antitoxin
JY387_RS05805	1231297..1232822	+	1526	Protein_1143	carboxylesterase/lipase family protein	-
JY387_RS05810	1232840..1233952	-	1113	WP_003405840.1	3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase	-
JY387_RS05815	1233962..1234219	-	258	WP_003405844.1	exodeoxyribonuclease VII small subunit	-
JY387_RS05820	1234209..1235456	-	1248	WP_206338152.1	exodeoxyribonuclease VII large subunit	-
JY387_RS05825	1235453..1236091	-	639	WP_003898730.1	lipid droplet-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-85)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11061.82 Da
• Isoelectric Point: 4.9371

>T195349 WP_206281013.1 NZ_CP071127:c1230967-1230656 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Nucleotide

• Length: 312 bp

>T195349 NZ_CP094624:5144001-5144738 [Bacillus thuringiensis]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAATCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCCATAAAACGATC
ATTAATTGCATCTAAAGCAAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCTGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCACAATTTAGTATTATCGTCCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11394.83 Da
• Isoelectric Point: 5.1236

>AT195349 WP_003405836.1 NZ_CP071127:c1231287-1230967 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Nucleotide

• Length: 321 bp

>AT195349 NZ_CP094624:5144851-5144979 [Bacillus thuringiensis]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: G0TGZ7