Detailed information of TA system TA195330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 363475..364046
• Replicon: chromosome
• Accession: NZ_CP071127
• Organism: Mycobacterium tuberculosis strain 120/26CAO

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: JY387_RS01585
• Protein ID: WP_003401560.1
• Coordinates: 363475..363777 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: JY387_RS01590
• Protein ID: WP_003401563.1
• Coordinates: 363825..364046 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY387_RS01565	358944..359747	-	804	WP_206280804.1	sulfotransferase	-
JY387_RS01570	359757..361154	-	1398	WP_003401544.1	sulfatase	-
JY387_RS01575	361333..363108	+	1776	WP_010886074.1	PE family protein	-
JY387_RS01580	363251..363478	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
JY387_RS01585	363475..363777	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
JY387_RS01590	363825..364046	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
JY387_RS01595	364043..364468	+	426	WP_003401566.1	PIN domain nuclease	-
JY387_RS01600	364604..365236	+	633	WP_206280805.1	TetR family transcriptional regulator	-
JY387_RS01605	365233..366141	+	909	WP_206280806.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T195330 WP_003401560.1 NZ_CP071127:363475-363777 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T195330 NZ_CP094541:c132677-132357 [Acinetobacter sp. YH16040_T]
ATGTTATTCATTGAAACCAGTGTTTTTACCAAGCAAATTAAAGAGCTTGTAAGTGATGAAGAGTATCGTCAGCTCCAGCA
AGATCTTTTGGTACAACCTGATAAAGGTGACTTAATCAAGAATGGTGGTGGAATTCGTAAAGTACGCTGTACTCAAGGCA
ATAAAGGTAAAAGCGGCGGCATCCGGGTGATCTATTATTGGGTCACAGAAGATGATCAGATATTTTTCCTGGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAAACATCGATCCTGCGTCAATTAGTAAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT195330 WP_003401563.1 NZ_CP071127:363825-364046 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT195330 NZ_CP094541:c132364-132092 [Acinetobacter sp. YH16040_T]
ATGGATAACAACTTATTTGATGACCTGGTTGCTTCAATCAAAGAAGCTGGTGCCATCAAACGTAATGAGATAAAGGCCAG
TCGAGTCACAGAGCTTGAATTGCCAGATATTAAAGAAGTACGTGAGAAAACTGGATTAACGCAAGCAGAGTTTGCTGCAC
GTTTGCATATTAGCGCGAGAACGCTACAGAACTGGGAACAAGGCCGACGCTATCCGACTGGTCCTGCAGCGACTTTAATT
CGCATTCTGGATGCCCATCCAACTTTGATCTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CG48