Detailed information of TA system TA195320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2643180..2643709
• Replicon: chromosome
• Accession: NZ_CP071102
• Organism: Staphylococcus aureus strain CI/BAC/25/13/W

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JYA70_RS13025
• Protein ID: WP_000621175.1
• Coordinates: 2643347..2643709 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JYA70_RS13020
• Protein ID: WP_000948331.1
• Coordinates: 2643180..2643350 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JYA70_RS12990 (2638216)	2638216..2638776	+	561	WP_001092417.1	K(+)-transporting ATPase subunit C	-
JYA70_RS12995 (2638985)	2638985..2639464	+	480	WP_001287085.1	hypothetical protein	-
JYA70_RS13000 (2639457)	2639457..2641040	+	1584	WP_001294627.1	PH domain-containing protein	-
JYA70_RS13005 (2641027)	2641027..2641518	+	492	WP_001205910.1	PH domain-containing protein	-
JYA70_RS13010 (2641522)	2641522..2641881	+	360	WP_000581197.1	holo-ACP synthase	-
JYA70_RS13015 (2641947)	2641947..2643095	+	1149	WP_001281154.1	alanine racemase	-
JYA70_RS13020 (2643180)	2643180..2643350	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JYA70_RS13025 (2643347)	2643347..2643709	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JYA70_RS13030 (2644058)	2644058..2645059	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JYA70_RS13035 (2645178)	2645178..2645504	+	327	WP_001052491.1	anti-sigma factor antagonist	-
JYA70_RS13040 (2645506)	2645506..2645985	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
JYA70_RS13045 (2645960)	2645960..2646730	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T195320 WP_000621175.1 NZ_CP071102:2643347-2643709 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T195320 NZ_CP094525:8315839-8316192 [Bradyrhizobium sp. ISRA463]
TTGGCAACGAAGTCTGGTAACTACTGTCCCGAGGCCGGCGACTTCGTCTGGATCGATCTTGATCCCACGATCGGCCACGA
ACAAAGCGGCCGACGACCGGCCATCGTATTATCGCCGCACAACTACAATGCCGCAGCCGGCCTTTGCGTGATCTGCCCAA
TCACAAGCAGCGTACGCGACTATCCCTTCGAGGCCAAGCTGCCCGACGGCGCCAGCTTTCACGGCGTCGTGCTCGCCGAC
CAACCCCGCAGCATCTCGTGGGAGAAGCGACCGATCCAGCATGCCGGACGAGCGCCTGACGTCCTGCTCACCGAAGTCCG
TGAGCGTTTGGCCGCGTTGCTCGGAATCGACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT195320 WP_000948331.1 NZ_CP071102:2643180-2643350 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT195320 NZ_CP094525:8315604-8315858 [Bradyrhizobium sp. ISRA463]
ATGAAGGTAGCATTTCAGAAGTGGGGCAACAGCCTGGCACTGAGAGTTCCAAAAGCATTCGCCGATGAAATCGGTGCCAG
CGACGGCAAGGCCGCCGAAATGACCGTGAGCGACGGCAAACTGGTGATCGAGATCGCGCGCTCGCCGCGGCGCAAGCGTC
GCTACGCACTCGACAAGCTGGTTGCAGGTATAACGCCGGACAACCGTCATGAAGAGGTTGATTGGGGGCGACCCATTGGC
AACGAAGTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1