Detailed information of TA system TA195296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2098164..2098693
• Replicon: chromosome
• Accession: NZ_CP071100
• Organism: Staphylococcus aureus strain PS/BAC/169/17/W

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JYA66_RS10340
• Protein ID: WP_000621175.1
• Coordinates: 2098164..2098526 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JYA66_RS10345
• Protein ID: WP_000948331.1
• Coordinates: 2098523..2098693 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JYA66_RS10320 (2095143)	2095143..2095913	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
JYA66_RS10325 (2095888)	2095888..2096367	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
JYA66_RS10330 (2096369)	2096369..2096695	-	327	WP_001052491.1	anti-sigma factor antagonist	-
JYA66_RS10335 (2096814)	2096814..2097815	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JYA66_RS10340 (2098164)	2098164..2098526	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JYA66_RS10345 (2098523)	2098523..2098693	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JYA66_RS10350 (2098778)	2098778..2099926	-	1149	WP_000130547.1	alanine racemase	-
JYA66_RS10355 (2099992)	2099992..2100351	-	360	WP_000581202.1	holo-ACP synthase	-
JYA66_RS10360 (2100355)	2100355..2100846	-	492	WP_001205912.1	PH domain-containing protein	-
JYA66_RS10365 (2100833)	2100833..2102416	-	1584	WP_001294620.1	PH domain-containing protein	-
JYA66_RS10370 (2102409)	2102409..2102888	-	480	WP_001287079.1	hypothetical protein	-
JYA66_RS10375 (2103097)	2103097..2103657	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T195296 WP_000621175.1 NZ_CP071100:c2098526-2098164 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T195296 NZ_CP094512:831128-831538 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT195296 WP_000948331.1 NZ_CP071100:c2098693-2098523 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT195296 NZ_CP094512:830881-831147 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1