Detailed information of TA system TA195295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1942272..1943048
• Replicon: chromosome
• Accession: NZ_CP071100
• Organism: Staphylococcus aureus strain PS/BAC/169/17/W

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: JYA66_RS09435
• Protein ID: WP_000031108.1
• Coordinates: 1942272..1942424 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: JYA66_RS09440
• Protein ID: WP_001251224.1
• Coordinates: 1942449..1943048 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JYA66_RS09420 (1938411)	1938411..1939232	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
JYA66_RS09425 (1939693)	1939693..1941078	-	1386	WP_000116224.1	class II fumarate hydratase	-
JYA66_RS09430 (1941274)	1941274..1941669	-	396	WP_000901021.1	hypothetical protein	-
JYA66_RS09435 (1942272)	1942272..1942424	-	153	WP_000031108.1	SAS053 family protein	Toxin
JYA66_RS09440 (1942449)	1942449..1943048	-	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
JYA66_RS09445 (1943206)	1943206..1943676	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
JYA66_RS09450 (1943681)	1943681..1944808	-	1128	WP_000379985.1	tRNA epoxyqueuosine(34) reductase QueG	-
JYA66_RS09455 (1944959)	1944959..1945681	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
JYA66_RS09460 (1945674)	1945674..1947131	-	1458	WP_089535760.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T195295 WP_000031108.1 NZ_CP071100:c1942424-1942272 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T195295 NZ_CP094512:350765-351112 [Klebsiella pneumoniae]
ATGTGGTATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT195295 WP_001251224.1 NZ_CP071100:c1943048-1942449 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT195295 NZ_CP094512:351112-351411 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4