Detailed information of TA system TA195286

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/PRK09907-MazE
• Location: 999504..1000075
• Replicon: chromosome
• Accession: NZ_CP071097
• Organism: Fusobacterium animalis strain THCT7A2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: R9RDI3
• Locus tag: JY398_RS04735
• Protein ID: WP_008798672.1
• Coordinates: 999752..1000075 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: R9REM8
• Locus tag: JY398_RS04730
• Protein ID: WP_016340052.1
• Coordinates: 999504..999758 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY398_RS04715 (JY398_04715)	994961..995380	-	420	WP_008693346.1	NfeD family protein	-
JY398_RS04720 (JY398_04720)	995622..997091	+	1470	WP_220298260.1	N-acetylglucosamine-specific PTS transporter subunit IIBC	-
JY398_RS04725 (JY398_04725)	997178..999244	+	2067	WP_220298261.1	elongation factor G	-
JY398_RS04730 (JY398_04730)	999504..999758	+	255	WP_016340052.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JY398_RS04735 (JY398_04735)	999752..1000075	+	324	WP_008798672.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JY398_RS04740 (JY398_04740)	1000110..1000367	+	258	WP_008701957.1	hypothetical protein	-
JY398_RS04745 (JY398_04745)	1000360..1000680	+	321	WP_008798671.1	type II toxin-antitoxin system YoeB family toxin	-
JY398_RS04750 (JY398_04750)	1000734..1001018	-	285	WP_005910587.1	ferredoxin family protein	-
JY398_RS04755 (JY398_04755)	1001021..1002316	-	1296	WP_220298262.1	FAD-dependent oxidoreductase	-
JY398_RS04760 (JY398_04760)	1002341..1003036	-	696	WP_158410143.1	ubiquinone/menaquinone biosynthesis methyltransferase	-
JY398_RS04765 (JY398_04765)	1003038..1003961	-	924	WP_220298263.1	prenyltransferase	-
JY398_RS04770 (JY398_04770)	1003958..1004938	-	981	WP_220298264.1	polyprenyl synthetase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-106)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12550.70 Da
• Isoelectric Point: 5.7723

>T195286 WP_008798672.1 NZ_CP071097:999752-1000075 [Fusobacterium animalis]
VVERGDIIIINFDPIKRHEQVGKRPVLVISNEKFYKIFKLAVILPITNNTKDFPFHVLLDNRTNIKGAILCEYLKTVDLE
ERKYNKVEELPEDLLEEVLEKVSLIFQ

Nucleotide

• Length: 324 bp

>T195286 NZ_CP094479:c4646422-4646081 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGAAGCTATGTCTGTCGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGTTGGTGCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCGGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTAAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 10005.53 Da
• Isoelectric Point: 6.3520

>AT195286 WP_016340052.1 NZ_CP071097:999504-999758 [Fusobacterium animalis]
MEVLRVQKWGNSQGIRLPKKILKDLGININDKIEISLNGEEIILKKIKKYTNLDDLFKDYKGDYRKDFDEFEFFGEAQGC
EFKW

Nucleotide

• Length: 255 bp

>AT195286 NZ_CP094479:c4646784-4646443 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATGTGGCATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA

Structures

Toxin

• AlphaFold DB: R9RDI3

Antitoxin

• AlphaFold DB: R9REM8