Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4887725..4888535 Replicon chromosome
Accession NZ_CP071086
Organism Klebsiella pneumoniae strain 3347689I

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag JY394_RS24340 Protein ID WP_002887280.1
Coordinates 4887725..4888258 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JY394_RS24345 Protein ID WP_002887278.1
Coordinates 4888269..4888535 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JY394_RS24335 (JY394_24335) 4886556..4887677 + 1122 WP_009309849.1 cupin domain-containing protein -
JY394_RS24340 (JY394_24340) 4887725..4888258 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
JY394_RS24345 (JY394_24345) 4888269..4888535 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
JY394_RS24350 (JY394_24350) 4888638..4890071 - 1434 WP_019724905.1 Cu(+)/Ag(+) sensor histidine kinase -
JY394_RS24355 (JY394_24355) 4890061..4890744 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
JY394_RS24360 (JY394_24360) 4890916..4892298 + 1383 WP_019724904.1 efflux transporter outer membrane subunit -
JY394_RS24365 (JY394_24365) 4892316..4892660 + 345 WP_025987660.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T195271 WP_002887280.1 NZ_CP071086:c4888258-4887725 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T195271 NZ_CP094468:774599-774796 [Faecalibacterium sp. I3-3-89]
GTGAAAAGCTACTCTTCCCGAGAGGTCATAAAGGCGCTCAAGGCCGACGGCTGGTACGAGGTCAACTGTGTGGGAAGCCA
CCATCAGTATAAGCACCCCACCAAGCCCGGCCGTGTCACCGTAAAAGACCCAGACAAAGATATTCCCCGGGCCACACTGA
ATCGTATCGAGCAGCAGTCCGGTCTCAAATTCCGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT195271 WP_002887278.1 NZ_CP071086:c4888535-4888269 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT195271 NZ_CP094468:774816-775226 [Faecalibacterium sp. I3-3-89]
ATGAAAAAGAATCTTCCCGACCGTTATTTCTATCCTGCCGTGTTCATCTATGAGGACGGGCAGGAGATCGCCGTCGATTT
TCCCGACCTCGGTGTTGCCACCAGCGGCACGTCTGAGGACGATGCTCTGCTCTCGGCCCGAGAACTGCTTGGCTGCGTGA
TGTGCGGGCTGGAGGAGGATGGCGAGCCTATCCCGGCGCCCTCCGCCCTGTCGGACATCCACCCCAAGGACAACGAGCGG
GTGGTGCTGGTCGATGCCTATATGCCCTCCGTCCGGCTGGCCAGCGTCAATCGCTCCGTCAACCGCACGGTCACTCTCCC
GGCGTGGCTCAACGCCGCAGCCCTTGAGCGCAATGTGAACTTCAGCCAAGTCCTTCAGGACGCTCTGAAGCATCAGCTCC
ATCTTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References