Detailed information of TA system TA195256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5857..6382
• Replicon: plasmid p3347684I_3
• Accession: NZ_CP071083
• Organism: Klebsiella pneumoniae strain 3347684I

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: JY393_RS26915
• Protein ID: WP_004197633.1
• Coordinates: 5857..6162 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: JY393_RS26920
• Protein ID: WP_004197642.1
• Coordinates: 6164..6382 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY393_RS26885 (JY393_26880)	1519..2145	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
JY393_RS27915	2142..2444	+	303	WP_004197636.1	hypothetical protein	-
JY393_RS26895 (JY393_26890)	2932..3726	-	795	WP_004197635.1	site-specific integrase	-
JY393_RS26900 (JY393_26895)	3924..4940	-	1017	WP_004197639.1	hypothetical protein	-
JY393_RS26905 (JY393_26900)	4937..5260	-	324	WP_004197641.1	hypothetical protein	-
JY393_RS26910 (JY393_26905)	5287..5688	-	402	WP_072199347.1	hypothetical protein	-
JY393_RS26915 (JY393_26910)	5857..6162	-	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
JY393_RS26920 (JY393_26915)	6164..6382	-	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
JY393_RS26925 (JY393_26920)	6978..7226	+	249	WP_019725036.1	hypothetical protein	-
JY393_RS26930 (JY393_26925)	7223..7795	+	573	WP_015065504.1	hypothetical protein	-
JY393_RS26935 (JY393_26930)	7826..8320	+	495	WP_009310053.1	hypothetical protein	-
JY393_RS26940 (JY393_26935)	8364..8732	+	369	WP_015065502.1	hypothetical protein	-
JY393_RS26945 (JY393_26940)	8766..8969	+	204	WP_019725040.1	HHA domain-containing protein	-
JY393_RS26950 (JY393_26945)	8983..9213	+	231	WP_019725034.1	hypothetical protein	-
JY393_RS26955 (JY393_26950)	9257..9451	-	195	WP_019725033.1	hypothetical protein	-
JY393_RS26960 (JY393_26955)	9885..10208	-	324	WP_019725650.1	hypothetical protein	-
JY393_RS26965 (JY393_26960)	10281..11276	-	996	WP_019725651.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IIa / qnrB1 / aac(6')-Ib-cr / blaOXA-1 / dfrA14 / blaCTX-M-15	-	1..64471	64471

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T195256 WP_004197633.1 NZ_CP071083:c6162-5857 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T195256 NZ_CP094460:674857-675270 [Burkholderia pyrrocinia]
ATGAGCGGGATTGAGTCGGCAAAATCATTCGTCGACAGCAATGTCGTGCTTTATCTGCTGTCCGGGGACGAAACCAAGGC
GAATCGGGCGGAGGCGCTGTTCCTTCGGCGGCCGACCATCAGTGTCCAGGTCCTGAACGAAGTCGCCAGCGTTTGCAGCC
GAAAATTGCGGATGCCGTGGAGCGACGTGGCGCAGTTTCTGGAACCGATCAGGAGCTTGTGCCATGTCGTACCGGTCACG
ATCGATACGCATGACCTGGCGCGAAGGCTGGCGGAGCGACATGGCTTGTCATTCTATGACGCGTGCATCGTCGCCGCCGC
GTCGCTCGAGGGCTGCGAGTACCTGTACACCGAGGACATGCACGATGGCCTGCGTGTGGATGGTGGACCGGTGCTGCGAA
ATCCGTTCGCCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT195256 WP_004197642.1 NZ_CP071083:c6382-6164 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT195256 NZ_CP094460:674637-674870 [Burkholderia pyrrocinia]
ATGCAGGTCGCAAAGTGGGGCAATAGCCTGGCCGTCCGCCTTCCCGCCACTGTGGTCGAAGCCCTGGAGCTCCGCGAAGG
GGACGACATCGAGATCGTGGTCGATAGCCCGCGCGTGTTTGCCGTCAGCCGCAAGCCGAGGGCCGATGAACTGCTGCAAC
GGTTGCGGCGTTTCAGGGGCAAGCTGCCCGCCGATTTCAAGTTCAGCCGGGACGATGCGAATGAGCGGGATTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5