195254

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5212429..5213054 Replicon chromosome
Accession NZ_CP071080
Organism Klebsiella pneumoniae strain 3347684I

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag JY393_RS25105 Protein ID WP_002882817.1
Coordinates 5212429..5212812 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JY393_RS25110 Protein ID WP_004150355.1
Coordinates 5212812..5213054 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JY393_RS25090 (5209795) 5209795..5210697 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JY393_RS25095 (5210694) 5210694..5211329 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JY393_RS25100 (5211326) 5211326..5212255 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JY393_RS25105 (5212429) 5212429..5212812 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
JY393_RS25110 (5212812) 5212812..5213054 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
JY393_RS25115 (5213259) 5213259..5214176 + 918 WP_004178029.1 alpha/beta hydrolase -
JY393_RS25120 (5214190) 5214190..5215131 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JY393_RS25125 (5215176) 5215176..5215613 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
JY393_RS25130 (5215610) 5215610..5216470 - 861 WP_004146232.1 virulence factor BrkB family protein -
JY393_RS25135 (5216464) 5216464..5217063 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T195254 WP_002882817.1 NZ_CP071080:c5212812-5212429 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T195254 NZ_CP094459:1814078-1814494 [Burkholderia pyrrocinia]
GTGACCACGCTCTACATGCTCGATACGAACATCTGTTCGTTCATCATGCGCGAGCGGCCGCCCGTGGTGCTGTCGCGGTT
GCAGTCGTGCGTCAACGCTCAGCACCGGATCGTCGTGTCGGCAATCACCTATGCCGAAATGCGGTTCGGCGCGGTCGGCA
GGAAAGCGTCGCCGAAGCATGCGGAACTCGTGACGGCGTTTGTTTCGCGGCTGGATGGCGTGCTGTCGTGGGATACGGCG
GCCGTCGACGCAACGGCCGCGATTCGCGCGGATCTCGCCGTGCGCGGCACGCCGATCGGCGCGAACGATGCGTCGATCGC
CGGGCACGCGATCGCGGCCGGCGCGGTGCTCGTCACGAACAACGGCCGCGAATTCGGCCGTGTTGCCGGGTTGTCGCTCG
AAGACTGGGCGGCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT195254 WP_004150355.1 NZ_CP071080:c5213054-5212812 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT195254 NZ_CP094459:1813818-1814081 [Burkholderia pyrrocinia]
ATGCGCACCGTTTCCATTTTCAAGAACGCCCGTAATCAGGCAATTCGCATCCCGAAGGACATGGAGTTCGAGGGGGTGAC
GGAACTCGAGATCCGCCGCGAGGGCGACACGCTGCTGCTGCGCCCCGTGCGCCCCACGTGGCTGTCGTTCGCGAACGAAC
CGCTGGCCGACGCCGATTTCCTCGCCGACCGCCCGGCCGTCATCGAATCCGGCCGCTTCGACCTGTCCGGTGACGCCGGC
GCGCCGACGGAGTCCGGCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References