Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | phd-doc/Doc-Phd |
| Location | 120631..121232 | Replicon | plasmid p3347558_2 |
| Accession | NZ_CP071075 | ||
| Organism | Escherichia coli strain 3347558 | ||
Toxin (Protein)
| Gene name | doc | Uniprot ID | V0AJ64 |
| Locus tag | JY392_RS26625 | Protein ID | WP_001216034.1 |
| Coordinates | 120852..121232 (+) | Length | 127 a.a. |
Antitoxin (Protein)
| Gene name | phd | Uniprot ID | U9YQH9 |
| Locus tag | JY392_RS26620 | Protein ID | WP_001190712.1 |
| Coordinates | 120631..120852 (+) | Length | 74 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JY392_RS26590 (JY392_26585) | 116401..116898 | - | 498 | WP_001514138.1 | heat resistance protein PsiE-GI | - |
| JY392_RS26595 (JY392_26590) | 116901..118610 | - | 1710 | WP_023326110.1 | heat resistance system K+/H+ antiporter KefB-GI | - |
| JY392_RS26600 (JY392_26595) | 118614..119054 | - | 441 | WP_000786815.1 | heat resistance system thioredoxin Trx-GI | - |
| JY392_RS26605 (JY392_26600) | 119044..119481 | - | 438 | Protein_127 | hypothetical protein | - |
| JY392_RS26615 (JY392_26610) | 120245..120448 | - | 204 | Protein_129 | type I restriction-modification system subunit M N-terminal domain-containing protein | - |
| JY392_RS26620 (JY392_26615) | 120631..120852 | + | 222 | WP_001190712.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| JY392_RS26625 (JY392_26620) | 120852..121232 | + | 381 | WP_001216034.1 | type II toxin-antitoxin system death-on-curing family toxin | Toxin |
| JY392_RS26630 (JY392_26625) | 121237..121416 | + | 180 | WP_001513661.1 | hypothetical protein | - |
| JY392_RS26635 (JY392_26630) | 121444..121722 | + | 279 | Protein_133 | pdcB | - |
| JY392_RS26640 (JY392_26635) | 121727..122140 | + | 414 | Protein_134 | integrase core domain-containing protein | - |
| JY392_RS26645 (JY392_26640) | 122090..122425 | - | 336 | WP_169329198.1 | type I deoxyribonuclease HsdR | - |
| JY392_RS26650 (JY392_26645) | 122635..123615 | - | 981 | WP_000019407.1 | IS5-like element IS5 family transposase | - |
| JY392_RS26655 (JY392_26650) | 123859..125262 | + | 1404 | WP_001373486.1 | S-methylmethionine permease | - |
| JY392_RS26660 (JY392_26655) | 125249..126181 | + | 933 | WP_023143441.1 | homocysteine S-methyltransferase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | tet(B) / dfrA14 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B | senB | 1..129523 | 129523 | |
| - | inside | IScluster/Tn | - | - | 119488..127012 | 7524 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 127 a.a. Molecular weight: 13615.32 Da Isoelectric Point: 5.1514
>T195231 WP_001216034.1 NZ_CP071075:120852-121232 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE
Download Length: 381 bp
>T195231 NZ_CP094443:2480880-2481146 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA
Antitoxin
Download Length: 74 a.a. Molecular weight: 8133.10 Da Isoelectric Point: 4.7708
>AT195231 WP_001190712.1 NZ_CP071075:120631-120852 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
Download Length: 222 bp
>AT195231 NZ_CP094443:2480629-2480880 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0AJ64 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CJB6 |