Detailed information of TA system TA195229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4782645..4783257
• Replicon: chromosome
• Accession: NZ_CP071073
• Organism: Escherichia coli strain 3347558

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: JY392_RS23250
• Protein ID: WP_000833473.1
• Coordinates: 4783072..4783257 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: JY392_RS23245
• Protein ID: WP_000499744.1
• Coordinates: 4782645..4783055 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY392_RS23230 (4778157)	4778157..4778972	-	816	WP_000891825.1	AraC family transcriptional regulator	-
JY392_RS23235 (4779198)	4779198..4780598	+	1401	WP_000204802.1	MFS transporter	-
JY392_RS23240 (4780609)	4780609..4782573	+	1965	WP_001026861.1	glycoside hydrolase family 127 protein	-
JY392_RS23245 (4782645)	4782645..4783055	-	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JY392_RS23250 (4783072)	4783072..4783257	-	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
JY392_RS23255 (4783535)	4783535..4785073	-	1539	WP_001309845.1	aldehyde dehydrogenase AldB	-
JY392_RS23260 (4785274)	4785274..4786425	-	1152	WP_000741501.1	L-threonine dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T195229 WP_000833473.1 NZ_CP071073:c4783257-4783072 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T195229 NZ_CP094443:2424182-2424448 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT195229 WP_000499744.1 NZ_CP071073:c4783055-4782645 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT195229 NZ_CP094443:2423925-2424182 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9