Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4033284..4033938 Replicon chromosome
Accession NZ_CP071073
Organism Escherichia coli strain 3347558

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag JY392_RS19525 Protein ID WP_000244769.1
Coordinates 4033284..4033691 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JY392_RS19530 Protein ID WP_000354046.1
Coordinates 4033672..4033938 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JY392_RS19505 (4029241) 4029241..4030974 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
JY392_RS19510 (4030980) 4030980..4031690 - 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JY392_RS19515 (4031715) 4031715..4032611 - 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
JY392_RS19520 (4032723) 4032723..4033244 + 522 WP_001055874.1 flavodoxin FldB -
JY392_RS19525 (4033284) 4033284..4033691 - 408 WP_000244769.1 protein YgfX Toxin
JY392_RS19530 (4033672) 4033672..4033938 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JY392_RS19535 (4034181) 4034181..4035161 + 981 WP_000886049.1 tRNA-modifying protein YgfZ -
JY392_RS19540 (4035238) 4035238..4035897 - 660 WP_000250274.1 hemolysin III family protein -
JY392_RS19545 (4036061) 4036061..4036372 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JY392_RS19550 (4036417) 4036417..4037850 + 1434 WP_001513393.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T195225 WP_000244769.1 NZ_CP071073:c4033691-4033284 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T195225 NZ_CP094443:c174951-174856 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT195225 WP_000354046.1 NZ_CP071073:c4033938-4033672 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT195225 NZ_CP094443:174771-174828 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References