Detailed information of TA system TA195216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2436343..2437931
• Replicon: chromosome
• Accession: NZ_CP071073
• Organism: Escherichia coli strain 3347558

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9YVT8
• Locus tag: JY392_RS11915
• Protein ID: WP_001125415.1
• Coordinates: 2436343..2437665 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JY392_RS11920
• Protein ID: WP_001296726.1
• Coordinates: 2437665..2437931 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY392_RS11875 (2431377)	2431377..2431949	-	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-
JY392_RS11880 (2432100)	2432100..2432252	-	153	WP_001322417.1	amidohydrolase family protein	-
JY392_RS11885 (2432242)	2432242..2432661	-	420	WP_001322416.1	amidohydrolase family protein	-
JY392_RS11890 (2432621)	2432621..2432776	-	156	WP_001322415.1	hypothetical protein	-
JY392_RS11895 (2432879)	2432879..2433826	-	948	WP_000854796.1	aldo/keto reductase	-
JY392_RS11900 (2433900)	2433900..2434589	-	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
JY392_RS11905 (2434598)	2434598..2434906	-	309	WP_001221046.1	hypothetical protein	-
JY392_RS11910 (2435046)	2435046..2435945	+	900	WP_001057776.1	LysR family transcriptional regulator	-
JY392_RS11915 (2436343)	2436343..2437665	-	1323	WP_001125415.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
JY392_RS11920 (2437665)	2437665..2437931	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49384.05 Da
• Isoelectric Point: 8.5830

>T195216 WP_001125415.1 NZ_CP071073:c2437665-2436343 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T195216 NZ_CP094361:2065106-2065198 [Bacillus subtilis]
ATGTCAACATTCCAGGCATTAATGCTAATGCTTGCAATCGGGTCGTTTATAATCGCCCTATTGACGTATATAGAAAAAAT
AGACCTCCCTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT195216 WP_001296726.1 NZ_CP071073:c2437931-2437665 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT195216 NZ_CP094361:c2065352-2065179 [Bacillus subtilis]
TTACAATACATTACAGTGCATTTCTTTAATGAGAAATGCATAAAATAAAAAAGACCGAGGTGCTACCAACACCCCGGCTC
TGTACAAAAAGTTGCCCATCAAAGGGCTTGCTCGATGTGGTTCTAGGTAGGCCTATCCCTTCAGGTTCTGAGGCTCAAGG
GAGGTCTATTTTTT

Structures

Toxin

• AlphaFold DB: U9YVT8