Detailed information of TA system TA195214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2337571..2338209
• Replicon: chromosome
• Accession: NZ_CP071073
• Organism: Escherichia coli strain 3347558

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: JY392_RS11470
• Protein ID: WP_000813797.1
• Coordinates: 2337571..2337747 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JY392_RS11475
• Protein ID: WP_076611057.1
• Coordinates: 2337793..2338209 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY392_RS11450 (2333193)	2333193..2334365	-	1173	WP_001236223.1	BenE family transporter YdcO	-
JY392_RS11455 (2334457)	2334457..2334993	+	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
JY392_RS11460 (2335066)	2335066..2337027	+	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JY392_RS11465 (2337119)	2337119..2337349	-	231	WP_000494244.1	YncJ family protein	-
JY392_RS11470 (2337571)	2337571..2337747	+	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JY392_RS11475 (2337793)	2337793..2338209	+	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JY392_RS11480 (2338288)	2338288..2339694	+	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
JY392_RS11485 (2339939)	2339939..2341084	+	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
JY392_RS11490 (2341102)	2341102..2342115	+	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
JY392_RS11495 (2342116)	2342116..2343057	+	942	WP_001251335.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T195214 WP_000813797.1 NZ_CP071073:2337571-2337747 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T195214 NZ_CP094361:518540-518890 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT195214 WP_076611057.1 NZ_CP071073:2337793-2338209 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT195214 NZ_CP094361:518254-518535 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A829J715