Detailed information of TA system TA195209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1942724..1943508
• Replicon: chromosome
• Accession: NZ_CP071073
• Organism: Escherichia coli strain 3347558

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: JY392_RS09355
• Protein ID: WP_000613626.1
• Coordinates: 1942724..1943218 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: JY392_RS09360
• Protein ID: WP_001110447.1
• Coordinates: 1943215..1943508 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY392_RS09335 (1937917)	1937917..1939014	+	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-
JY392_RS09340 (1939014)	1939014..1939955	+	942	WP_001309406.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
JY392_RS09345 (1940021)	1940021..1941664	+	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
JY392_RS09350 (1941676)	1941676..1942629	+	954	WP_202851812.1	flagellar hook-associated protein FlgL	-
JY392_RS09355 (1942724)	1942724..1943218	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
JY392_RS09360 (1943215)	1943215..1943508	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
JY392_RS09365 (1943641)	1943641..1946826	-	3186	WP_000827427.1	ribonuclease E	-
JY392_RS09370 (1947399)	1947399..1948358	+	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T195209 WP_000613626.1 NZ_CP071073:c1943218-1942724 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T195209 NZ_CP094359:1848948-1849349 [Acidithiobacillus sp. YTS05]
GTGATCAACTACCTGCTGGATACCAACATCTGCATTTTCACCATCAAGCGCAAGCCGCCGGAGATTCGGGAACAGTTCCG
CCGGCATCACGGACAAATGGCCATCAGCGCGATCACCTTGATGGAGCTGATCTATGGTGCGGAGAAGTCACAGGCACCAG
AGCGCAACCTGGCGGAGATCGAACATTTCACGGCGAGATTGGAGCTTCTCGAATTCGGGAGTGAGGCCGCGAAGCATACC
GGGCAGATCCGCGCCGAGCTAACCCGGATTGGTAAGCCTATCAGTCCATATGATCAGATGATCGCGGGCCACGCCCGAAG
CCGGGGATTGATCGTCGTGACCAACAACCTGCGGGAGTTCGCACGGGTTCCCGGGTTGCGCGTCGTGGATTGGGTATCCT
GA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT195209 WP_001110447.1 NZ_CP071073:c1943508-1943215 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT195209 NZ_CP094359:1848721-1848951 [Acidithiobacillus sp. YTS05]
ATGCAAAAAGCCACTATCTTTCTGAGTAACCGCAGTCAGGCGGTACGTTTACCCAAAGCCATCGCCTTTCCCGCTGATGT
GAAACGCGTGGAAGTGCTCACCGTGGGCCGGACGCGGATTCTGATCCCTGAAGGGGAGTCCTGGGACAGCTGGTTCGATA
ACGAAGGGGTCAGTGCAGATTTCATGGAAGATCGGGAGCAACCCGCTGATCAGGCCCGCGAGTCCTTGTGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6