Detailed information of TA system TA195208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1694766..1695471
• Replicon: chromosome
• Accession: NZ_CP071073
• Organism: Escherichia coli strain 3347558

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: JY392_RS08185
• Protein ID: WP_000539521.1
• Coordinates: 1695085..1695471 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JY392_RS08180
• Protein ID: WP_001280945.1
• Coordinates: 1694766..1695095 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY392_RS08165 (1689923)	1689923..1690834	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
JY392_RS08170 (1691012)	1691012..1693360	+	2349	Protein_1601	cyclic diguanylate phosphodiesterase	-
JY392_RS08175 (1693368)	1693368..1694696	+	1329	WP_000086879.1	GGDEF domain-containing protein	-
JY392_RS08180 (1694766)	1694766..1695095	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JY392_RS08185 (1695085)	1695085..1695471	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JY392_RS08190 (1695697)	1695697..1697022	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JY392_RS08195 (1697235)	1697235..1697618	+	384	WP_000497137.1	biofilm formation regulator BssR	-
JY392_RS08200 (1697729)	1697729..1698844	+	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
JY392_RS08205 (1698841)	1698841..1699467	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T195208 WP_000539521.1 NZ_CP071073:c1695471-1695085 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T195208 NZ_CP094359:c1641526-1641245 [Acidithiobacillus sp. YTS05]
ATGCGGAGGATTGAGTACACCGGCCAATTCAAGCGCGACTACAAGCGTGAGGCGAGGGGGCATCACCGTGCCACGCTCGA
AGCGGATCTGCTGCCCGTCGTCAAAGCCCTGGCCTGCGACCAGCCGCTGGAGCCGCGCCATCACGATCATGCCCTGACTG
GCGATTGGAAAGATCATCGGGACTGTCATGTTAAGCCCGACCTAGTGTTGATCTATCGGAAACCCGATAACGAAGTGTTG
CAGCTCGTGCGTATTAGCTCGCATAGCGAGCTAGGGCTGTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT195208 WP_001280945.1 NZ_CP071073:c1695095-1694766 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT195208 NZ_CP094359:c1641776-1641513 [Acidithiobacillus sp. YTS05]
ATGTCTGCAAACGCCGTTGTCCGTGCTCGCATTGACGAGCATATCAAAGAAGAAGCATCAGCCGTTCTGGCGGCGATGGG
TCTTACTGTGTCCGATGCCTTTCGCATCATGCTGACCCGCATTGCGCGAGAGAAGGCATTGCCGTTTGAGCCCCTGGTGC
CGAACGAAACCACCATCGCCGCCATACGTGAAGCCCGCGCCGGCAACCTCAAGAGCTTCGATAGCGTTGAGGCGCTCATG
GCGGACTTGCATGCGGAGGATTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6