Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 314432..314929 Replicon chromosome
Accession NZ_CP071073
Organism Escherichia coli strain 3347558

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JY392_RS01545 Protein ID WP_001173343.1
Coordinates 314756..314929 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JY392_RS01540 Protein ID WP_000398619.1
Coordinates 314432..314728 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JY392_RS01520 (311161) 311161..311880 - 720 WP_000611288.1 two-component system response regulator DcuR -
JY392_RS01525 (311877) 311877..313508 - 1632 WP_001216477.1 sensor histidine kinase -
JY392_RS01530 (313689) 313689..313919 + 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
JY392_RS01535 (313931) 313931..314203 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
JY392_RS01540 (314432) 314432..314728 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JY392_RS01545 (314756) 314756..314929 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JY392_RS01550 (315047) 315047..316564 - 1518 WP_000003806.1 lysine--tRNA ligase -
JY392_RS01555 (316801) 316801..318258 - 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T195198 WP_001173343.1 NZ_CP071073:314756-314929 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T195198 NZ_CP094351:5104476-5105120 [Pseudomonas orientalis]
ATGATTTATAACAACCTGAATCCTGAAAAAGCGCTGATCTGGCGAATCGTGCATCGCGACAATCTGCCTTGGATTCTGGA
CAACGGCCTGCACTGCGCCAGCTCAGAGGTGCAGGCCGCGCAATATGTGGACATAGGCAATGTTGATTTAATCGACAAAC
GACGCACGCGCTGTGTCCCCATTGCTCCTTCAGGCTTTTTGTCAGACTACGTTCCCTTCTATTTCACACCCTTCTCTGTG
ATGATGAAAAATATTCACTCTGGCTGGAGCGTTCAACGACGCAACAATGACGAGATCGTGATTCTGGTCTCCAGCCTCCA
CCGCGTCGCCGAGCTTGGCCTACCGTTCGTCTTCACAAACGCACATGCGTACCCGGATTGGACAGACTATTACAGCGACC
TGGCGAATCTCCACGAGATCGATTGGTCTATCCTTCAACGGCGTGACTTCAAACGTGACCCCGATGACCCTCGCAAAATG
GAGCGTTATCAGGCTGAAGCGCTGATTCACCACCACTTACCGATTACAGGACTCCTTGGCATCATGTGTTACACCGATGC
GATGAAAGAACGCATAGAGCAGGACGTCGCCGCAAGAGGCCTGACGCTGCCTGTACATGCTCGTCCGGGATGGTATTTCC
CATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT195198 WP_000398619.1 NZ_CP071073:314432-314728 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT195198 NZ_CP094351:5105117-5106211 [Pseudomonas orientalis]
ATGATCAGATTCACCCAGGGCAACCTTCTGCAGGCCAACGCCGAAGTCCTCGTCAACACGGTGAATACCGTGGGCGTGAT
GGGCAAGGGCATCGCGCTGATGTTCAAAGAGCGTTTTGCAGAAAACTATCGCTTGTACGCTGCTGCCTGTAAAGCTGGCG
AGGTAGAGACCGGAAAAGTGCACGTAACTGCGGTCAACGAACTGGACGGTCCTCGCTGGATCGTTAACTTCCCGACCAAA
CGTCACTGGCGATCACCATCGCAGATGGCATGGATAACCGAAGGCCTGCACGACCTCCGTCGTTTTCTGATTGAAAACCA
TGTGAAGTCAGTGGCGGTTCCTCCGTTAGGGGCCGGTAACGGTGGTCTGAAATGGCCGGACGTTCGCGAACAGATCGAGG
ATGTCTTGAGCGATCTGGATGTCGATGTGTTGGTTTTTGAGCCATCCAATCAGTATCTGAATGTCGCCAAACGCAGCGGT
GTAGAACAGCTTACGCCTGCGCGCGCGCTCATTGCGGAACTGGTTCGACGCTACTGGGTGTTAGGTATGGAATGCAGCCT
GCTGGAGATTCAGAAACTGGCTTGGTTCCTGGAGCGCGCAATTGAGAAGCGGCCAGGCCTCAAAAACCCACTGGATCTTA
AGTTTGTTGCTCATAAGTACGGGCCGTATGCCAACCGGTTGGAACACCTGCTTAACAATCTGGACGGTAGTTATCTGCAT
TGCGATAAGCGCATCAGCGATGCGGAAAACAGTGATGTGATCTGGTTTGACCAAGACCGCAGAGCCTTTCTACAAACCTA
TCTCAGAACACAGGCCAAGGAATATTCCCAGGCCCTGGAGCTTACCGCGGAGTTGATCGATGGCTTTGAATCACCCTTCG
GCATGGAGCTATTGGCAACAGTCGACTGGCTGCTGACCCAGGAAGATGTAGCCCCAACCGTTCCGGCTGTACGTGAGGCA
CTGAAACGATGGAATGGCGGTGTCGCTGCAGCCGCTCGCAAAAACAAGCTGTTTGACGATCAAGCACTTGATATTGCGCT
TAAACGGCTCACCTCCAGCAGCTTCAGCCGTCAAATGACTTCCTGTCATATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References