Detailed information of TA system TA195197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 7514..8157
• Replicon: plasmid p3347089II_2
• Accession: NZ_CP071072
• Organism: Citrobacter sedlakii strain 3347689II

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q84A06
• Locus tag: JY391_RS23500
• Protein ID: WP_000754566.1
• Coordinates: 7741..8157 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: JY391_RS23495
• Protein ID: WP_001261276.1
• Coordinates: 7514..7744 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY391_RS23460 (JY391_23460)	2633..2902	+	270	WP_000339857.1	hypothetical protein	-
JY391_RS23465 (JY391_23465)	3079..3945	-	867	WP_004118283.1	replication initiation protein	-
JY391_RS23470 (JY391_23470)	4475..4579	+	105	WP_032409716.1	hypothetical protein	-
JY391_RS23475 (JY391_23475)	4708..4965	+	258	WP_000764642.1	hypothetical protein	-
JY391_RS23480 (JY391_23480)	5023..5799	-	777	WP_000015958.1	site-specific integrase	-
JY391_RS23485 (JY391_23485)	5796..6539	-	744	WP_000129823.1	hypothetical protein	-
JY391_RS23490 (JY391_23490)	6590..6940	-	351	WP_000493378.1	hypothetical protein	-
JY391_RS23495 (JY391_23495)	7514..7744	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JY391_RS23500 (JY391_23500)	7741..8157	+	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	Toxin
JY391_RS23505 (JY391_23505)	8361..9217	+	857	Protein_13	IS3-like element ISEc15 family transposase	-
JY391_RS23510 (JY391_23510)	9499..10575	+	1077	WP_000227969.1	IS110 family transposase	-
JY391_RS23515 (JY391_23515)	10595..10696	-	102	Protein_15	Tn3 family transposase	-
JY391_RS23520 (JY391_23520)	11216..11299	+	84	Protein_16	SOS response-associated peptidase	-
JY391_RS23875	11357..11428	-	72	Protein_17	hypothetical protein	-
JY391_RS23525 (JY391_23525)	11462..12649	-	1188	Protein_18	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ant(3'')-Ia / dfrA1 / aac(6')-Ib-cr / blaOXA-1 / catB3 / sul1 / mph(A)	-	1..44104	44104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15006.52 Da
• Isoelectric Point: 9.2957

>T195197 WP_000754566.1 NZ_CP071072:7741-8157 [Citrobacter sedlakii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T195197 NZ_CP094351:c4425676-4425278 [Pseudomonas orientalis]
ATGCTCAAGTTCATGCTCGATACCAACATCTGTATCTTCACAATCAAAAACAGACCCCAGCAGGTGCGCGAAACCTTCAG
AAAGCACTATGGCCAACTAGCTATCAGTACAGTGACCCTGATGGAGCTGATTTACGGCGCGGAAAAATCACCTAACCCAG
AGCGAAACTTGGCTGATGTCGAAGGCTTTGCAGCACGTCTGGAAGTTTTGAAATACGACCAGGATGCCGCTATGCACACT
GGCCAACTTCGCGCTGAGCTAGCGAGAGCTGGTACTCCGATAGGCTCGTATGACCAGATGATCGCTGGGCATGCCCGTTC
GCAGGGCCTGATTATGGTCACTAACAACCGGCGCGAGTTCGACCGCGTGCCAGGTTTGCGGGTAGAAGATTGGGTGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT195197 WP_001261276.1 NZ_CP071072:7514-7744 [Citrobacter sedlakii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT195197 NZ_CP094351:c4425906-4425676 [Pseudomonas orientalis]
ATGGACCAAGGTTCTGTATTTCAAAGCAACCGCAGCCAAGCTGTACGTTTGCCAAAGGCTGCTGCGTTGCCGGATGACGT
AAAACGTGTAGATGTCGTCTCAATCGGTAGGGCCCGAATCATCACCCCAGCCGGTGAGTCATGGGACAGCTGGTTCGATG
GCGAGGGTGTCACGCCGGACTTCATGAACGACCGAGATCAGCCTGCTGACCAAGAGCGGGAATCTTTTTAA

Structures

Toxin

• AlphaFold DB: A0A387K1G3

Antitoxin

• AlphaFold DB: A0A387K023