Detailed information of TA system TA195184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 348573..349159
• Replicon: chromosome
• Accession: NZ_CP071070
• Organism: Citrobacter sedlakii strain 3347689II

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: JY391_RS01635
• Protein ID: WP_211935133.1
• Coordinates: 348791..349159 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A0V9J767
• Locus tag: JY391_RS01630
• Protein ID: WP_060571155.1
• Coordinates: 348573..348794 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY391_RS01605 (JY391_01605)	343579..344688	+	1110	WP_181822399.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
JY391_RS01610 (JY391_01610)	344750..345676	+	927	WP_042286511.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
JY391_RS01615 (JY391_01615)	345673..346950	+	1278	WP_181822400.1	branched chain amino acid ABC transporter permease LivM	-
JY391_RS01620 (JY391_01620)	346947..347714	+	768	WP_042286506.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
JY391_RS01625 (JY391_01625)	347716..348429	+	714	WP_181822401.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
JY391_RS01630 (JY391_01630)	348573..348794	+	222	WP_060571155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JY391_RS01635 (JY391_01635)	348791..349159	+	369	WP_211935133.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
JY391_RS01640 (JY391_01640)	349402..350718	+	1317	WP_181822402.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
JY391_RS01645 (JY391_01645)	350817..351704	+	888	WP_042286499.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
JY391_RS01650 (JY391_01650)	351701..352546	+	846	WP_042286496.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
JY391_RS01655 (JY391_01655)	352548..353618	+	1071	WP_181822404.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	345673..354361	8688

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-52)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13531.90 Da
• Isoelectric Point: 9.0542

>T195184 WP_211935133.1 NZ_CP071070:348791-349159 [Citrobacter sedlakii]
MTLQFISAEEIVLFHDKLLSITPGVAGMADPGRAEALLYRVLNKYEHEGVTDIRILAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLKANPDFVAMTVEAAAGRLSLEQIVQRLRC

Nucleotide

• Length: 369 bp

>T195184 NZ_CP094343:5777056-5777331 [Pseudomonas sp. M1]
GTGCTATCGATCATCTGGCGCAGGAGTGCCAGTGATGACCTCGCGACCATTCTCGCCTATATAGCCAACGAGGATCCCCA
AGCAGCAAGGCGCCTCAAGGAACGCGTGGAGTCTGCCGTGCTGCCGTTGGCCCAGCATCCCTACCTTTACCGTCATGGAC
GAGTACCCGGCACACGAGAAGTCGTGATCCACCCCAACTACATCCTGGTTTACCGGATCGACGCTGAATGCATCGAAGTA
GTCAGCGTCCTGCATTCGCGCCAGGAATATCCCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8023.02 Da
• Isoelectric Point: 5.6579

>AT195184 WP_060571155.1 NZ_CP071070:348573-348794 [Citrobacter sedlakii]
MRTMNYSEARQNLASALDSAATGTPVTITRRGHKPAVIISAEEFERYQAAKLDAEFDAIMGIHGSEIRELADK

Nucleotide

• Length: 222 bp

>AT195184 NZ_CP094343:5776862-5777056 [Pseudomonas sp. M1]
ATGAGCGCCCGACTTTCCCCCATAGTCTCCGAGTTCGAAACCCCGGAGCAGGCAGCCAGCCATGATCGCTGGTTCCGCCA
AAAGGTGCAGAAGTCCCTTACAGACAACCAACCAGGGCGTCCCCATGACGAGGTAATGGTCGACATGGACGCCATCATTG
CCCAGGCCGAAGCCCGGCGCCAGGGCAACGACTAG

Structures

Antitoxin

• AlphaFold DB: A0A0V9J767