Detailed information of TA system TA195182

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 59638..60357
• Replicon: chromosome
• Accession: NZ_CP071070
• Organism: Citrobacter sedlakii strain 3347689II

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: JY391_RS00295
• Protein ID: WP_096080170.1
• Coordinates: 59638..59958 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JY391_RS00300
• Protein ID: WP_096080169.1
• Coordinates: 59992..60357 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY391_RS00270 (JY391_00270)	55474..56583	+	1110	WP_211935064.1	D-glucosaminate-6-phosphate ammonia lyase	-
JY391_RS00275 (JY391_00275)	56570..57313	+	744	WP_042287106.1	2-dehydro-3-deoxy-phosphogluconate aldolase	-
JY391_RS00280 (JY391_00280)	57397..57702	+	306	Protein_55	MetQ/NlpA family ABC transporter substrate-binding protein	-
JY391_RS00285 (JY391_00285)	57729..58631	-	903	WP_042287101.1	carboxylate/amino acid/amine transporter	-
JY391_RS00290 (JY391_00290)	58821..59339	+	519	WP_060569994.1	anti-virulence regulator CigR family protein	-
JY391_RS00295 (JY391_00295)	59638..59958	-	321	WP_096080170.1	TA system toxin CbtA family protein	Toxin
JY391_RS00300 (JY391_00300)	59992..60357	-	366	WP_096080169.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JY391_RS00305 (JY391_00305)	60389..60610	-	222	WP_096080168.1	DUF987 domain-containing protein	-
JY391_RS00310 (JY391_00310)	60619..61089	-	471	WP_096080167.1	DNA repair protein RadC	-
JY391_RS00315 (JY391_00315)	61160..61981	-	822	WP_096080166.1	DUF932 domain-containing protein	-
JY391_RS00320 (JY391_00320)	62056..62484	-	429	WP_096080165.1	hypothetical protein	-
JY391_RS00325 (JY391_00325)	62520..62699	-	180	WP_096080164.1	hypothetical protein	-
JY391_RS00330 (JY391_00330)	62750..63553	-	804	WP_096080163.1	hypothetical protein	-
JY391_RS00335 (JY391_00335)	63573..64007	-	435	WP_149463744.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-104)

Antitoxin

• Antitoxin CbeA (18-116)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12216.77 Da
• Isoelectric Point: 6.2076

>T195182 WP_096080170.1 NZ_CP071070:c59958-59638 [Citrobacter sedlakii]
MHISTVPATVTVSSRLSPVQVWQKLLTYILEHHYGLTINDTPFSNDTTIQEHIEAGVNLTDAVNFLVERFDLVRIDQKGF
SWQDQEHWITSLDVHRAQFNLGLKRS

Nucleotide

• Length: 321 bp

>T195182 NZ_CP094343:5410283-5410570 [Pseudomonas sp. M1]
GTGCGCGTACTTGAGTGGCGGGAAGCTGCCCGCGCCGACCTGCTGGCGATTGTTGACTACATCTCGGACGACAACCCCGA
TGCCGCGCAGCGGTTGAAGGACGACATCGAGGCCAAGGCCGCGAAGCTGCCGGAGCGCCCGAAGCTCTACCGGCCTGGCC
GCGTGGCCGGCACGCGGGAGATGGTCGTGCGTGCGAACTACGTGGTGGTCTACATGGAGGACACCCGCGCGGTGTCCATC
CTGCGCGTGTTGCACGCCGCGCAGCAGTGGCCGCCGGCACGGGAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13546.27 Da
• Isoelectric Point: 6.8425

>AT195182 WP_096080169.1 NZ_CP071070:c60357-59992 [Citrobacter sedlakii]
MQSTTLSGSSTENASCLQWGLKRNIKPCFGARLVQEGNRLYFLTDRAGFDGSFSDDEALRLDQAFPLMMKQLERMLTTGE
LDPRSQHCVTRHHNGLTCEADTLGSHGYVYIAIYPQSALTR

Nucleotide

• Length: 366 bp

>AT195182 NZ_CP094343:5409997-5410296 [Pseudomonas sp. M1]
ATGGCCGCACAGACTTCGATGCTGCACATCCGTGTGGACGATAAGCTCAAGGCCGATGCCGCCGAGAAGCTCGCCGGGGT
CGGCCTCACCGTTTCGGACGCCGTGCGCATCCTGCTGACCCGTGTGGTCAAGGAAGGCGCGCTGCCGGCTGGCCTCACGG
CGGACCCGGAGGCCTACGATGCCTGGTTCCGGGCGAAGGTCCAGGAGGCGCTGGCCGACACACGGCCGCCGGTGCCGCAC
CAGCAGGTCATGGACGAGGCGCAGGCCCTGATCGACAGGAAGCGGCGTGCGCGTACTTGA