Detailed information of TA system TA195181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 39209..40782
• Replicon: plasmid pHS08-091
• Accession: NZ_CP071064
• Organism: Enterobacter mori strain 08-091

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JY395_RS22690
• Protein ID: WP_215231262.1
• Coordinates: 39478..40782 (+)
• Length: 435 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JY395_RS22685
• Protein ID: WP_215231261.1
• Coordinates: 39209..39478 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JY395_RS22665 (JY395_22650)	34675..36084	+	1410	WP_244999863.1	MFS transporter	-
JY395_RS22670 (JY395_22655)	36175..37206	+	1032	WP_215231258.1	ABC transporter substrate-binding protein	-
JY395_RS22675 (JY395_22660)	37241..38251	+	1011	WP_215231259.1	iron ABC transporter permease	-
JY395_RS22680 (JY395_22665)	38238..38960	+	723	WP_215231260.1	ABC transporter ATP-binding protein	-
JY395_RS22685 (JY395_22670)	39209..39478	+	270	WP_215231261.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JY395_RS22690 (JY395_22675)	39478..40782	+	1305	WP_215231262.1	type II toxin-antitoxin system HipA family toxin	Toxin
JY395_RS22695 (JY395_22680)	41087..41443	+	357	WP_215231263.1	hypothetical protein	-
JY395_RS22700 (JY395_22685)	41675..42631	+	957	WP_215231264.1	zincin-like metallopeptidase domain-containing protein	-
JY395_RS22705 (JY395_22690)	42954..43850	-	897	WP_215231265.1	LysR family transcriptional regulator	-
JY395_RS22710 (JY395_22695)	44319..44783	-	465	WP_215231266.1	transglycosylase SLT domain-containing protein	-
JY395_RS22715 (JY395_22700)	45168..45548	+	381	WP_086524752.1	relaxosome protein TraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR	1..213374	213374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-67)

Sequences

Toxin

Protein

• Length: 435 a.a.
• Molecular weight: 48855.69 Da
• Isoelectric Point: 5.3124

>T195181 WP_215231262.1 NZ_CP071064:39478-40782 [Enterobacter mori]
MPVLTTWMNNERVGELTKHANGAHTFRYDREWLYNRYARPISLSLPLQFGTITSDAVINYFDNLLPDNPAIRDRIVRRYQ
TPSRQPFDLLTEIGRDSVGAVTLLPQGEHTPAPVMSWDILNETRLEEILSSYQSDIPLGMINGEDDFRISVAGAQEKTAL
LKIDRDWCIPRGITPTSHIIKLPIGEIHQPSATLDLSESVDNEYFCLALAAALGIDVPNAEIINAGDVRALAVERFDRRW
NREHTMLLRLPQEDLCQVFGLPSSVKYESDGGPGIPEIMEFLLGSSNALQDRFSFIKFQVFQWLLGATDGHAKNFSVFIE
AGGSYRLTPFYDIISAFPVLGGRGLHIGDLKLAMSLKATKGRKYEINQIYPRHFFALADEVNFRKSEMDEILDFFATHVP
AALQRVSESLPDGFSVEVVESITRSMTKLHARLV

Nucleotide

• Length: 1305 bp

>T195181 NZ_CP094343:5379878-5380225 [Pseudomonas sp. M1]
ATGTCCAAGCTCGTCATCCGTCTCACCGATACCGCAGAACAAAGCATCGAAGACCAAGTACACCACTTGGCTCAATTCCA
AGGTTCGCAGGCTGCACTCCAGTCAGTGCTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCTCTTACACCCCAAGGTT
ACCCTATCAGCCAGCAGGCAAGCCTTTTGGGGGTGTTGAACTATCGCGAACTCAATACCGGCCCTTATCGCGTCTTTTAC
GAACTACACGAAGAGCAAGGCGAGGTCGCGGTTATCCTGGTCTTACGCCAGAAGCAGAGTGTGGAACAGCAATTGATTCG
CTACTGCTTGGTGGGGCCTATCGGGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10218.68 Da
• Isoelectric Point: 8.4731

>AT195181 WP_215231261.1 NZ_CP071064:39209-39478 [Enterobacter mori]
VSTLAIYSPKQLANYLKLTRQKHGFTQAELAARIGIKQGTISNFENNPDKTTIKTFFLLIQALELSLVLYDKDEMRNGDP
DSRQKEMDW

Nucleotide

• Length: 270 bp

>AT195181 NZ_CP094343:5379617-5379868 [Pseudomonas sp. M1]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGCCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACACAGAA
CGGTGTTCCTGCCTACGTGATTGAGTCATATGCAGAACGAAAGCAACGTGACGAAGCAATTGCGTTGGTGAAGCTGCTTG
CGATCGGCTCTCGTCAATACGCAGAAGGCAAACATCGCTCTGTAGATGATCTGAAAGCTCGTCTTTCCAGGAGGTTCGAT
CAACCAGAATAA