Detailed information of TA system TA195139

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 513258..513894
• Replicon: chromosome
• Accession: NZ_CP071043
• Organism: Bacillus subtilis isolate ELA2001105

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: JS609_RS02625
• Protein ID: WP_003156187.1
• Coordinates: 513544..513894 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: JS609_RS02620
• Protein ID: WP_003225183.1
• Coordinates: 513258..513539 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JS609_RS02600 (JS609_00513)	509618..510217	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
JS609_RS02605 (JS609_00514)	510312..510677	+	366	WP_015252768.1	holo-ACP synthase	-
JS609_RS02610 (JS609_00515)	510843..511859	+	1017	WP_072557167.1	outer membrane lipoprotein carrier protein LolA	-
JS609_RS02615 (JS609_00516)	511973..513142	+	1170	WP_015252766.1	alanine racemase	-
JS609_RS02620 (JS609_00517)	513258..513539	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
JS609_RS02625 (JS609_00518)	513544..513894	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
JS609_RS02630 (JS609_00519)	514009..514833	+	825	WP_015252765.1	RsbT co-antagonist protein RsbRA	-
JS609_RS02635 (JS609_00520)	514838..515203	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
JS609_RS02640 (JS609_00521)	515207..515608	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
JS609_RS02645 (JS609_00522)	515620..516627	+	1008	WP_015252763.1	phosphoserine phosphatase RsbU	-
JS609_RS02650 (JS609_00523)	516689..517018	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
JS609_RS02655 (JS609_00524)	517015..517497	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
JS609_RS02660 (JS609_00525)	517463..518251	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
JS609_RS02665 (JS609_00526)	518251..518850	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T195139 WP_003156187.1 NZ_CP071043:513544-513894 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T195139 NZ_CP094332:c4202182-4201898 [Salmonella enterica subsp. enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT195139 WP_003225183.1 NZ_CP071043:513258-513539 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT195139 NZ_CP094332:c4202414-4202172 [Salmonella enterica subsp. enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32