195139

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 513258..513894 Replicon chromosome
Accession NZ_CP071043
Organism Bacillus subtilis isolate ELA2001105

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag JS609_RS02625 Protein ID WP_003156187.1
Coordinates 513544..513894 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag JS609_RS02620 Protein ID WP_003225183.1
Coordinates 513258..513539 (+) Length 94 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JS609_RS02600 (JS609_00513) 509618..510217 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
JS609_RS02605 (JS609_00514) 510312..510677 + 366 WP_015252768.1 holo-ACP synthase -
JS609_RS02610 (JS609_00515) 510843..511859 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
JS609_RS02615 (JS609_00516) 511973..513142 + 1170 WP_015252766.1 alanine racemase -
JS609_RS02620 (JS609_00517) 513258..513539 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
JS609_RS02625 (JS609_00518) 513544..513894 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
JS609_RS02630 (JS609_00519) 514009..514833 + 825 WP_015252765.1 RsbT co-antagonist protein RsbRA -
JS609_RS02635 (JS609_00520) 514838..515203 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
JS609_RS02640 (JS609_00521) 515207..515608 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
JS609_RS02645 (JS609_00522) 515620..516627 + 1008 WP_015252763.1 phosphoserine phosphatase RsbU -
JS609_RS02650 (JS609_00523) 516689..517018 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
JS609_RS02655 (JS609_00524) 517015..517497 + 483 WP_003234299.1 anti-sigma B factor RsbW -
JS609_RS02660 (JS609_00525) 517463..518251 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
JS609_RS02665 (JS609_00526) 518251..518850 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-111)

Antitoxin

(12-45)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T195139 WP_003156187.1 NZ_CP071043:513544-513894 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T195139 NZ_CP094332:c4202182-4201898 [Salmonella enterica subsp. enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT195139 WP_003225183.1 NZ_CP071043:513258-513539 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT195139 NZ_CP094332:c4202414-4202172 [Salmonella enterica subsp. enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8


Antitoxin

Source ID Structure
AlphaFold DB G4NU32

References