Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17461..18104 Replicon plasmid pKP-1PI_FIB
Accession NZ_CP071030
Organism Klebsiella pneumoniae strain KP-1Pi

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag HZC88_RS28645 Protein ID WP_000754566.1
Coordinates 17688..18104 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HZC88_RS28640 Protein ID WP_001261276.1
Coordinates 17461..17691 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZC88_RS28605 (HZC88_28505) 12647..13120 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
HZC88_RS28610 (HZC88_28510) 13212..13442 + 231 WP_011977773.1 hypothetical protein -
HZC88_RS28615 (HZC88_28515) 14334..15116 - 783 WP_004152340.1 site-specific integrase -
HZC88_RS28620 (HZC88_28520) 15116..15448 - 333 WP_004152339.1 hypothetical protein -
HZC88_RS28625 (HZC88_28525) 15455..15853 - 399 WP_004171440.1 hypothetical protein -
HZC88_RS28630 (HZC88_28530) 15879..16208 - 330 WP_004152337.1 hypothetical protein -
HZC88_RS28635 (HZC88_28535) 16236..16544 - 309 WP_004152336.1 hypothetical protein -
HZC88_RS28640 (HZC88_28540) 17461..17691 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HZC88_RS28645 (HZC88_28545) 17688..18104 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
HZC88_RS28650 (HZC88_28550) 18308..19164 + 857 Protein_18 IS3-like element ISEc15 family transposase -
HZC88_RS28655 (HZC88_28555) 19446..20522 + 1077 WP_000227969.1 IS110 family transposase -
HZC88_RS28660 (HZC88_28560) 20542..20643 - 102 Protein_20 Tn3 family transposase -
HZC88_RS28665 (HZC88_28565) 21269..21595 - 327 WP_004201125.1 C2H2-type zinc finger protein -
HZC88_RS28670 (HZC88_28570) 21777..22058 + 282 WP_001568067.1 hypothetical protein -
HZC88_RS28675 (HZC88_28575) 22112..22723 + 612 WP_000176305.1 DUF2913 family protein -
HZC88_RS28680 (HZC88_28580) 22720..22830 - 111 Protein_24 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaNDM-1 / aph(3')-VI / qnrS1 / blaCTX-M-15 / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr - 1..54064 54064
- flank IS/Tn - - 11259..12527 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T195136 WP_000754566.1 NZ_CP071030:17688-18104 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T195136 NZ_CP094332:3450150-3450368 [Salmonella enterica subsp. enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT195136 WP_001261276.1 NZ_CP071030:17461-17691 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT195136 NZ_CP094332:3449748-3450122 [Salmonella enterica subsp. enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References