Detailed information of TA system TA195134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 154431..155101
• Replicon: plasmid pKP-1PI_HIB-FIB
• Accession: NZ_CP071028
• Organism: Klebsiella pneumoniae strain KP-1Pi

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: HZC88_RS27035
• Protein ID: WP_004213072.1
• Coordinates: 154431..154874 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: HZC88_RS27040
• Protein ID: WP_004213073.1
• Coordinates: 154871..155101 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZC88_RS27000 (HZC88_26910)	149838..150113	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
HZC88_RS27005 (HZC88_26915)	150176..150667	+	492	WP_011251327.1	DM13 domain-containing protein	-
HZC88_RS27010 (HZC88_26920)	150716..151636	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
HZC88_RS27015 (HZC88_26925)	151727..152130	+	404	Protein_170	GAF domain-containing protein	-
HZC88_RS27020 (HZC88_26930)	152649..153287	-	639	Protein_171	mucoid phenotype regulator RmpA2	-
HZC88_RS27025 (HZC88_26935)	153704..154008	+	305	Protein_172	transposase	-
HZC88_RS27030	154031..154282	-	252	WP_186987481.1	hypothetical protein	-
HZC88_RS27035 (HZC88_26945)	154431..154874	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
HZC88_RS27040 (HZC88_26950)	154871..155101	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HZC88_RS27045 (HZC88_26955)	155709..156842	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
HZC88_RS27050 (HZC88_26960)	156858..157151	+	294	WP_004213076.1	hypothetical protein	-
HZC88_RS27055 (HZC88_26965)	157141..157347	-	207	WP_004213077.1	hypothetical protein	-
HZC88_RS27060 (HZC88_26970)	157699..157989	+	291	WP_004213078.1	hypothetical protein	-
HZC88_RS27065 (HZC88_26975)	157979..158878	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / mph(A) / sul1 / qacE / dfrA5 / mph(E) / msr(E) / armA / sul2 / blaCTX-M-15	rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..340568	340568

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T195134 WP_004213072.1 NZ_CP071028:c154874-154431 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T195134 NZ_CP094332:2019306-2019409 [Salmonella enterica subsp. enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT195134 WP_004213073.1 NZ_CP071028:c155101-154871 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT195134 NZ_CP094332:c2019411-2019266 [Salmonella enterica subsp. enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620