Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5282518..5283143 Replicon chromosome
Accession NZ_CP071027
Organism Klebsiella pneumoniae strain KP-1Pi

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HZC88_RS25745 Protein ID WP_002882817.1
Coordinates 5282518..5282901 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HZC88_RS25750 Protein ID WP_004150355.1
Coordinates 5282901..5283143 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZC88_RS25730 (5279884) 5279884..5280786 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HZC88_RS25735 (5280783) 5280783..5281418 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HZC88_RS25740 (5281415) 5281415..5282344 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HZC88_RS25745 (5282518) 5282518..5282901 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HZC88_RS25750 (5282901) 5282901..5283143 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HZC88_RS25755 (5283348) 5283348..5284265 + 918 WP_021466676.1 alpha/beta hydrolase -
HZC88_RS25760 (5284279) 5284279..5285220 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HZC88_RS25765 (5285265) 5285265..5285702 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HZC88_RS25770 (5285699) 5285699..5286559 - 861 WP_004146232.1 virulence factor BrkB family protein -
HZC88_RS25775 (5286553) 5286553..5287152 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T195132 WP_002882817.1 NZ_CP071027:c5282901-5282518 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T195132 NZ_CP094332:849442-849840 [Salmonella enterica subsp. enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT195132 WP_004150355.1 NZ_CP071027:c5283143-5282901 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT195132 NZ_CP094332:849215-849442 [Salmonella enterica subsp. enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References