Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1468824..1469560 Replicon chromosome
Accession NZ_CP071027
Organism Klebsiella pneumoniae strain KP-1Pi

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag HZC88_RS07245 Protein ID WP_032433360.1
Coordinates 1469078..1469560 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HZC88_RS07240 Protein ID WP_003026799.1
Coordinates 1468824..1469090 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZC88_RS07215 (1464470) 1464470..1465609 + 1140 WP_032433368.1 mannitol dehydrogenase -
HZC88_RS07220 (1465638) 1465638..1466300 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
HZC88_RS07225 (1466281) 1466281..1467288 + 1008 WP_280954017.1 dihydroxyacetone kinase subunit DhaK -
HZC88_RS07230 (1467306) 1467306..1467938 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
HZC88_RS07235 (1467948) 1467948..1468511 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
HZC88_RS07240 (1468824) 1468824..1469090 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HZC88_RS07245 (1469078) 1469078..1469560 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
HZC88_RS07250 (1469760) 1469760..1471163 + 1404 WP_001272054.1 ISNCY-like element ISKpn21 family transposase -
HZC88_RS07255 (1471192) 1471192..1471824 - 633 WP_001567369.1 hypothetical protein -
HZC88_RS07260 (1472204) 1472204..1472803 - 600 WP_064472304.1 helix-turn-helix transcriptional regulator -
HZC88_RS07265 (1473016) 1473016..1473960 - 945 WP_077254249.1 fimbrial protein -
HZC88_RS07270 (1473972) 1473972..1474550 - 579 WP_032432061.1 type 1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1436769..1482786 46017
- flank IS/Tn - - 1469760..1471163 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T195118 WP_032433360.1 NZ_CP071027:1469078-1469560 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T195118 NZ_CP094323:c1804570-1804313 [Edwardsiella anguillarum]
ATGCCAGAGATTGATGCCCTGATGGGGCTAAGCTTTGTGATGTATTTCTTCGACAATCAAACTCACAGTTTGCCGCATGT
CCATGTGAAATACGGCGAATTTGAGCTGGTTATCGGGATCGACTCAGGAGAGGCCTTGGAGGGTTACCTTCCCCCTAAAA
AGAGGAAACTGGCCGAGTCACATATTAAGGCCAATAAAACCAGGCTACTGGCTATGTGGCAGATGGCGGTTAATGGGATG
CATCCTGGGAAACTGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT195118 WP_003026799.1 NZ_CP071027:1468824-1469090 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT195118 NZ_CP094323:c1804303-1803791 [Edwardsiella anguillarum]
ATGTTGAAAATTATTGATGTGGATGTGGTCGGCGACCATGTTATCGAGGTCGAGTTTAGCGACGGCTTCCGTGGTAGGGC
TGATTTAACCGCGCTGTTCAGCAAACCGCCCTTCTCTGCGATCGCCGACTTTAACCGCTTTTCCCTGACGGCCAGCGGCG
TGCTGAACTGGGGAGATGCCGAGCTTTCTGCCGATACGGTTAGGCGCATGAGCAAAGGCGCAGTGGTATCGGCATCCTCA
CGCTCACTCATGCCAGAAAATGTAGAAGCGATCCTCCGCCAGGCCACCTGGGAATCCATGAGCGAGGGGCGCCCGGATAT
TTTGCAAGCCGCGTTAAGGGGATACGCGGAGCAGCTGGGCCACGCGGACGTCATCAAAAGGGCCGGGATTGCCAGCCGCA
GCAGTGCATACAAAACGCTATCGCCATCAACGAACCCCAGCTTTAAATCACTGGCAAAGATCAGCGGCGCGATTTTGGCT
ATCGTGCGTGAAAATAACGCGCAGCACGGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References