Detailed information of TA system TA195033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 45932..47555
• Replicon: plasmid pRIVM_C019181_1
• Accession: NZ_CP071016
• Organism: Enterobacter hormaechei strain RIVM_C019181

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: C1IUI3
• Locus tag: JQL71_RS22610
• Protein ID: WP_000088044.1
• Coordinates: 45932..47257 (-)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6M3PBR7
• Locus tag: JQL71_RS22615
• Protein ID: WP_012477377.1
• Coordinates: 47262..47555 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQL71_RS22580	41918..42775	-	858	WP_000784387.1	HNH endonuclease	-
JQL71_RS22585	42791..43099	-	309	WP_001224686.1	hypothetical protein	-
JQL71_RS22590	43648..44073	-	426	WP_000278471.1	hypothetical protein	-
JQL71_RS22595	44326..45141	-	816	WP_001641519.1	HNH endonuclease	-
JQL71_RS22600	45154..45564	-	411	WP_000985909.1	hypothetical protein	-
JQL71_RS22605	45665..45871	-	207	WP_000134171.1	hypothetical protein	-
JQL71_RS22610	45932..47257	-	1326	WP_000088044.1	type II toxin-antitoxin system HipA family toxin	Toxin
JQL71_RS22615	47262..47555	-	294	WP_012477377.1	helix-turn-helix transcriptional regulator	Antitoxin
JQL71_RS22620	47956..49344	-	1389	WP_000083579.1	hypothetical protein	-
JQL71_RS22625	49349..49846	-	498	WP_000340139.1	membrane protein	-
JQL71_RS22630	50049..50705	-	657	WP_000462754.1	trypsin-like serine protease	-
JQL71_RS22635	51364..51756	-	393	WP_000341066.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-9 / mph(E) / msr(E) / blaACC-1 / blaVIM-1 / ant(3'')-Ia / qacE / sul1	-	1..316079	316079

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50333.32 Da
• Isoelectric Point: 6.5044

>T195033 WP_000088044.1 NZ_CP071016:c47257-45932 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T195033 NZ_CP094289:895008-895358 [Georgenia sp. TF02-10]
TTGATGCGCCGCGGTGAGATCCGCCTGACCGACCTCGATCCGGTCCGTGGTAGCGAGGCGAACAAGCGCCGGCCCGCCGT
CATCGTCAGCAACGACCGCGCCAACGCCGTCGCCGAGCGCCTGGGGCGCGGCGTGGTGACCATCGTTCCGATCACCAGCA
GCGTTGCGCGGATATACCCGTTCCAGACGCTGCTCCCGGCCGCGGCCACCGGACTGCGGGTGGACTCCAAGGCCCAGGCC
GAGCAGATCCGGTCGGTCGCCGTCGAGCGCATCGGACCGGTCGTGGGACGGCTGCCGCCGTCCCGGATGACCGCGCTTGC
CGACGCCATCCGCCTGCACCTACAGCTGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10860.30 Da
• Isoelectric Point: 10.3134

>AT195033 WP_012477377.1 NZ_CP071016:c47555-47262 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

>AT195033 NZ_CP094289:894794-895024 [Georgenia sp. TF02-10]
GTGAAGCTGAGCGTGAGCCTGTCCGCCGACGAGGTGGCCTCGCTGGACAAGTACGCCCGGGCCACCGGGCTGAGGTCCCG
GTCCGCAGTCGTGCAGCATGCCATCAGGCTGCTGGGCGACCCAGACCTCGAGCAGGACTACGCGGCGGCGTGGGACGAGT
GGGAGTCTTCCGGAGAGAGCTCGGCCTGGGACGCCACCGCCGGCGACGGGCTGGTTGATGCGCCGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A2S1XXW1

Antitoxin

• AlphaFold DB: A0A6M3PBR7