Detailed information of TA system TA195001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 111103..112726
• Replicon: plasmid pRIVM_C034177_1
• Accession: NZ_CP071012
• Organism: Enterobacter hormaechei strain RIVM_C034177

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: C1IUI3
• Locus tag: JQL78_RS23075
• Protein ID: WP_000088044.1
• Coordinates: 111401..112726 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A6M3PBR7
• Locus tag: JQL78_RS23070
• Protein ID: WP_012477377.1
• Coordinates: 111103..111396 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQL78_RS23050	106902..107294	+	393	WP_000341066.1	hypothetical protein	-
JQL78_RS23055	107953..108609	+	657	WP_000462754.1	trypsin-like serine protease	-
JQL78_RS23060	108812..109309	+	498	WP_000340139.1	membrane protein	-
JQL78_RS23065	109314..110702	+	1389	WP_000083579.1	hypothetical protein	-
JQL78_RS23070	111103..111396	+	294	WP_012477377.1	helix-turn-helix transcriptional regulator	Antitoxin
JQL78_RS23075	111401..112726	+	1326	WP_000088044.1	type II toxin-antitoxin system HipA family toxin	Toxin
JQL78_RS23080	112787..112993	+	207	WP_000134171.1	hypothetical protein	-
JQL78_RS23085	113094..113504	+	411	WP_000985909.1	hypothetical protein	-
JQL78_RS23090	113517..114332	+	816	WP_001641519.1	HNH endonuclease	-
JQL78_RS23095	114585..115010	+	426	WP_000278471.1	hypothetical protein	-
JQL78_RS23100	115559..115867	+	309	WP_001224686.1	hypothetical protein	-
JQL78_RS23105	115883..116740	+	858	WP_000784387.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaACC-1 / msr(E) / mph(E) / mcr-9 / sul1 / qacE / ant(3'')-Ia / blaVIM-1	-	1..316077	316077

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50333.32 Da
• Isoelectric Point: 6.5044

>T195001 WP_000088044.1 NZ_CP071012:111401-112726 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T195001 NZ_CP094271:c30367-30086 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10860.30 Da
• Isoelectric Point: 10.3134

>AT195001 WP_012477377.1 NZ_CP071012:111103-111396 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Nucleotide

• Length: 294 bp

>AT195001 NZ_CP094271:c30608-30357 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A2S1XXW1

Antitoxin

• AlphaFold DB: A0A6M3PBR7