Detailed information of TA system TA194956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2688651..2689180
• Replicon: chromosome
• Accession: NZ_CP070991
• Organism: Staphylococcus aureus strain CBS2016-05

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JYA46_RS13385
• Protein ID: WP_000621175.1
• Coordinates: 2688818..2689180 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JYA46_RS13380
• Protein ID: WP_000948331.1
• Coordinates: 2688651..2688821 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JYA46_RS13350 (2683687)	2683687..2684247	+	561	WP_063652345.1	K(+)-transporting ATPase subunit C	-
JYA46_RS13355 (2684456)	2684456..2684935	+	480	WP_072491785.1	hypothetical protein	-
JYA46_RS13360 (2684928)	2684928..2686511	+	1584	WP_072491784.1	PH domain-containing protein	-
JYA46_RS13365 (2686498)	2686498..2686989	+	492	WP_001205919.1	PH domain-containing protein	-
JYA46_RS13370 (2686993)	2686993..2687352	+	360	WP_000581198.1	holo-ACP synthase	-
JYA46_RS13375 (2687418)	2687418..2688566	+	1149	WP_072491783.1	alanine racemase	-
JYA46_RS13380 (2688651)	2688651..2688821	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JYA46_RS13385 (2688818)	2688818..2689180	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JYA46_RS13390 (2689529)	2689529..2690530	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JYA46_RS13395 (2690649)	2690649..2690975	+	327	WP_001052491.1	anti-sigma factor antagonist	-
JYA46_RS13400 (2690977)	2690977..2691456	+	480	WP_098818138.1	anti-sigma B factor RsbW	-
JYA46_RS13405 (2691431)	2691431..2692201	+	771	WP_072491781.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T194956 WP_000621175.1 NZ_CP070991:2688818-2689180 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T194956 NZ_CP094238:c3480327-3480031 [Comamonas sp. 7D-2]
ATGTTCACCATCAAGCGAACCGCTGAATTTGATGCATGGCTATTCGGCCTGCGCGACACCATGACGCGCATCCGCCTTGC
CAAGCGACTGGACAAGGTGCAGCGTGGCCCGCTTGGAGATACAAAGCCCGTGGGTGATGGCGTGCATGAGCTGCGCGAGG
ACTTCGGCCCTGGCTGGTGCATGTACTACGTGCAGCACGGTGATGTGCTGGTGGTGATGCTGGGCGGAGGGGACAAGTCC
ACCCAGCAGGCCGACATTCAGGCCGCGATTGCTTTGTCCAAGACCCTGGAGGACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT194956 WP_000948331.1 NZ_CP070991:2688651-2688821 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT194956 NZ_CP094238:c3480028-3479732 [Comamonas sp. 7D-2]
ATGACCAAGATTGCAGACCTGCCCACCTTTGATATGGCAGAGCACCTGAAAACCGATGAAGACCTGGCTGCCTACCTATC
CTTGGTGCTGGAAGAAGACGACCCCGCAGAGCTGGCCAACGCTCTGGGTGTGATTGCCCGCGCCCGTGGCATGACCGAGA
TTGCCAGCGCCAGCGGCCTGACCCGCGAGGCTCTTTACAAGGCGCTACGCCCTGGCAGCCAGCCGCGCTTTGACACCATC
CGCCGGGTCTGTGGTGCCCTTGGGGTTCGCTTGGTGGCCCAGCCTATCCACGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1