Detailed information of TA system TA194933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2107380..2107909
• Replicon: chromosome
• Accession: NZ_CP070986
• Organism: Staphylococcus aureus strain WBG8287

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JYH18_RS10275
• Protein ID: WP_000621175.1
• Coordinates: 2107380..2107742 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JYH18_RS10280
• Protein ID: WP_000948331.1
• Coordinates: 2107739..2107909 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JYH18_RS10255	2104359..2105129	-	771	WP_001041108.1	RNA polymerase sigma factor SigB	-
JYH18_RS10260	2105104..2105583	-	480	WP_001190830.1	anti-sigma B factor RsbW	-
JYH18_RS10265	2105585..2105911	-	327	WP_001052491.1	anti-sigma factor antagonist	-
JYH18_RS10270	2106030..2107031	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JYH18_RS10275	2107380..2107742	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JYH18_RS10280	2107739..2107909	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JYH18_RS10285	2107994..2109142	-	1149	WP_001281154.1	alanine racemase	-
JYH18_RS10290	2109208..2109567	-	360	WP_000581197.1	holo-ACP synthase	-
JYH18_RS10295	2109571..2110062	-	492	WP_001205912.1	PH domain-containing protein	-
JYH18_RS10300	2110049..2111632	-	1584	WP_001294626.1	PH domain-containing protein	-
JYH18_RS10305	2111625..2112104	-	480	WP_001287078.1	hypothetical protein	-
JYH18_RS10310	2112313..2112873	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T194933 WP_000621175.1 NZ_CP070986:c2107742-2107380 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T194933 NZ_CP094231:180307-180579 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT194933 WP_000948331.1 NZ_CP070986:c2107909-2107739 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT194933 NZ_CP094231:180044-180307 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1