Detailed information of TA system TA194919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2091734..2092263
• Replicon: chromosome
• Accession: NZ_CP070983
• Organism: Staphylococcus aureus strain WBG8366

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JX573_RS10365
• Protein ID: WP_000621177.1
• Coordinates: 2091734..2092096 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: JX573_RS10370
• Protein ID: WP_000948331.1
• Coordinates: 2092093..2092263 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JX573_RS10345	2088712..2089482	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
JX573_RS10350	2089457..2089936	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
JX573_RS10355	2089938..2090264	-	327	WP_001052491.1	anti-sigma factor antagonist	-
JX573_RS10360	2090383..2091384	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
JX573_RS10365	2091734..2092096	-	363	WP_000621177.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JX573_RS10370	2092093..2092263	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
JX573_RS10375	2092348..2093496	-	1149	WP_001281147.1	alanine racemase	-
JX573_RS10380	2093562..2093921	-	360	WP_000581202.1	holo-ACP synthase	-
JX573_RS10385	2093925..2094416	-	492	WP_001205910.1	PH domain-containing protein	-
JX573_RS10390	2094403..2095986	-	1584	WP_001294627.1	PH domain-containing protein	-
JX573_RS10395	2095979..2096458	-	480	WP_001287078.1	hypothetical protein	-
JX573_RS10400	2096667..2097227	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13471.72 Da
• Isoelectric Point: 10.1654

>T194919 WP_000621177.1 NZ_CP070983:c2092096-2091734 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNTVAHQKN

Nucleotide

• Length: 363 bp

>T194919 NZ_CP094227:4521674-4522117 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT194919 WP_000948331.1 NZ_CP070983:c2092263-2092093 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT194919 NZ_CP094227:4521447-4521677 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1